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dc.contributor.authorFleming, Jeanen_US
dc.contributor.authorTan, O.en_US
dc.contributor.authorDong, Y.en_US
dc.contributor.authorClements, J.en_US
dc.date.accessioned2017-05-03T15:00:53Z
dc.date.available2017-05-03T15:00:53Z
dc.date.issued2006en_US
dc.date.modified2007-09-06
dc.identifier.urihttp://hdl.handle.net/10072/14787
dc.description.abstractEpithelial ovarian cancer (EOC) remains a lethal disease because few specific biomarkers exist for early stage detection. Many serous EOCs arise from fluid-filled "inclusion" cysts in the ovary, lined with cells from ovarian surface epithelium (OSE). Kallikrein serine protease 4 (KLK4) is poorly expressed in OSE, but up regulated in serous EOC cell lines and tumours and is thus a potential biomarker for serous EOC. Mouse ovaries subjected to incessant ovulation form ovarian inclusion cysts which are similar histologically to human benign serous cystadenomas. We investigated Klk4 protein expression in mouse ovaries with high lifetime ovulation number, to determine if Klk4 protein was up-regulated during inclusion cyst development and whether Klk4 expression correlated with histopathology in the mouse ovary. Incessant ovulation was induced for up to 12-months by keeping Swiss Webster mice in cages divided by a screen. Breeding stock of the same age were controls. Mouse Klk4 expression was measured by immunohistochemistry with three anti-hKLK4 anti-peptide antibodies, directed to the N- terminus (Ab01), mid region (Ab03) and C-terminus (Ab05) of human KLK4. Cytoplasmic immunoreactivity was detected with Ab01 and Ab03 and nuclear immunoreactivity with Ab05, in stromal cells, luteal cells, granulosa cells of some apoptotic antral follicles and in some, but not all oocytes in primordial or primary follicles, in ovaries from incessantly ovulated mice or breeders. No immunoreactivity was detected in OSE. Cysts were observed in ovaries from both breeders and incessantly ovulated mice. Immunoreactivity with Ab01/03 was inconsistent in cyst cells, although some dysplastic cells showed basal cytoplasmic staining with Ab03. Strong nuclear staining was observed with Ab05 in the majority of cyst cells, with weaker staining in rete ovarii. We conclude from these preliminary studies that Klk4 expression is up-regulated in inclusion cysts observed in cystic mouse ovaries.en_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherASN Events Pty Ltden_US
dc.publisher.placeBalnarring VIC 3926en_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofconferencenameAnnual meeting of the Society for Reproductive Biologyen_US
dc.relation.ispartofconferencetitleProceedings of the Society for Reproductive Biologyen_US
dc.relation.ispartofdatefrom2006-08-20en_US
dc.relation.ispartofdateto2006-08-23en_US
dc.relation.ispartoflocationGold Coast, Australiaen_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchcode270106en_US
dc.subject.fieldofresearchcode270108en_US
dc.titleKallikrein 4 expression in mouse ovaries with inclusion cysts.en_US
dc.typeConference outputen_US
dc.type.descriptionE3 - Conference Publications (Extract Paper)en_US
dc.type.codeE - Conference Publicationsen_US
gro.facultyGriffith Sciences, School of Natural Sciencesen_US
gro.date.issued2006
gro.hasfulltextNo Full Text


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