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dc.contributor.authorNawaratna, Sujeevi SK
dc.contributor.authorGobert, Geoffrey N
dc.contributor.authorWillis, Charlene
dc.contributor.authorMulvenna, Jason
dc.contributor.authorHofmann, Andreas
dc.contributor.authorMcManus, Donald P
dc.contributor.authorJones, Malcolm K
dc.date.accessioned2017-09-01T01:33:20Z
dc.date.available2017-09-01T01:33:20Z
dc.date.issued2015
dc.identifier.issn2045-2322
dc.identifier.doi10.1038/srep15069
dc.identifier.urihttp://hdl.handle.net/10072/152476
dc.description.abstractOur previously reported gene atlasing of schistosome tissues revealed transcripts that were highly enriched in the digestive tract of Schistosoma mansoni. From these, we selected two candidates, Sm-LAMP and Sm-NPC2 for testing as vaccine targets. The two molecules were selected on the basis of relatively high expression in the gastrodermis, their potentially important biological function, divergence from homologous molecules of the host and possible apical membrane expression in the gastrodermis. Bacterially expressed recombinant peptides corresponding to regions excluding trans-membrane domains of the selected vaccine targets were used in blinded vaccine trials in CBA mice using alum-CpG as adjuvant. Vaccine trials using the recombinant insoluble Sm-LAMP protein showed 16–25% significant reduction in total worm burden. Faecal egg count reduction was 52% and 60% in two trials, respectively, with similar results for the solubly expressed protein. Liver egg burden was reduced significantly (20% and 38%) with an insoluble recombinant Sm-LAMP in two trials, but not with the soluble recombinant form. Parasite fecundity was not affected by either Sm-LAMP protein preparations in the trials. It is concluded that Sm-LAMP may provide limited protection towards S. mansoni infections but could be used in combination with other vaccine candidates, to provide more comprehensive protection.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Publishing Group
dc.publisher.placeLondon
dc.relation.ispartofpagefrom15069-1
dc.relation.ispartofpageto15069-12
dc.relation.ispartofjournalScientific Reports
dc.relation.ispartofvolume5
dc.subject.fieldofresearchMedical parasitology
dc.subject.fieldofresearchcode320704
dc.titleLysosome-associated membrane glycoprotein (LAMP) – preliminary study on a hidden antigen target for vaccination against schistosomiasis
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2015. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
gro.hasfulltextFull Text
gro.griffith.authorHofmann, Andreas
gro.griffith.authorNawaratna, Sujeevi S.
gro.griffith.authorWillis, Charlene
gro.griffith.authorGobert, Geoffrey N.
gro.griffith.authorJones, Malcolm K.


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