Show simple item record

dc.contributor.authorF. Brown, Nat
dc.contributor.authorA. Vallance, Bruce
dc.contributor.authorK. Coombes, Brian
dc.contributor.authorVandez, Yanet
dc.contributor.authorA. Coburn, Bryan
dc.contributor.authorBrett Finlay, B.
dc.date.accessioned2017-05-03T12:25:16Z
dc.date.available2017-05-03T12:25:16Z
dc.date.issued2005
dc.date.modified2008-11-19T02:55:10Z
dc.identifier.issn15537366
dc.identifier.doi10.1371/journal.ppat.0010032
dc.identifier.urihttp://hdl.handle.net/10072/15337
dc.description.abstractSalmonella enterica serovar Typhimurium is a facultative intracellular pathogen that causes disease in mice that resembles human typhoid. Typhoid pathogenesis consists of distinct phases in the intestine and a subsequent systemic phase in which bacteria replicate in macrophages of the liver and spleen. The type III secretion system encoded by Salmonella pathogenicity island 2 (SPI-2) is a major virulence factor contributing to the systemic phase of typhoid pathogenesis. Understanding how pathogens regulate virulence mechanisms in response to the environment, including different host tissues, is key to our understanding of pathogenesis. A recombinase-based in vivo expression technology system was developed to assess SPI-2 expression during murine typhoid. SPI-2 expression was detectable at very early times in bacteria that were resident in the lumen of the ileum and was independent of active bacterial invasion of the epithelium. We also provide direct evidence for the regulation of SPI-2 by the Salmonella transcription factors ompR and ssrB in vivo. Together these results demonstrate that SPI-2 expression precedes penetration of the intestinal epithelium. This induction of expression precedes any documented SPI-2-dependent phases of typhoid and may be involved in preparing Salmonella to successfully resist the antimicrobial environment encountered within macrophages.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent250975 bytes
dc.format.extent44011 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.languageEnglish
dc.language.isoeng
dc.publisherPublic Library of Science
dc.publisher.placeUSA
dc.publisher.urihttp://www.plos.org/
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom252
dc.relation.ispartofpageto258
dc.relation.ispartofissue3
dc.relation.ispartofjournalPLoS Pathogens
dc.relation.ispartofvolume1
dc.rights.retentionY
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchImmunology
dc.subject.fieldofresearchMedical Microbiology
dc.subject.fieldofresearchcode0605
dc.subject.fieldofresearchcode1107
dc.subject.fieldofresearchcode1108
dc.titleSalmonella pathogenicity island 2 is expressed prior to penetrating the intestine
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://www.plos.org/journals/license.html
gro.rights.copyright© 2005 Brown et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)
gro.date.issued2005
gro.hasfulltextFull Text
gro.griffith.authorBrown, Nathaniel F.


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record