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dc.contributor.authorRemmerbach, Torsten
dc.contributor.authorG. Brinckmann, Ute
dc.contributor.authorHemprich, Alexander
dc.contributor.authorChekol, M.
dc.contributor.authorKuhndel, K.
dc.contributor.authorLiebert, Uwe Gerd
dc.date.accessioned2017-05-03T15:18:43Z
dc.date.available2017-05-03T15:18:43Z
dc.date.issued2004
dc.date.modified2009-01-15T06:18:02Z
dc.identifier.issn13866532
dc.identifier.doi10.1016/j.jcv.2003.12.011
dc.identifier.urihttp://hdl.handle.net/10072/15441
dc.description.abstractBACKGROUND AND OBJECTIVES: Human papillomaviruses (HPV) are the causal agent for the development of carcinomas in the cervix uteri and further pathological changes of the skin including mucosa, particularly warts, condylomas and dysplasias. Therefore, we investigated the efficacy of different consensus primers pairs for HPV detection by PCR using brushed samples from the oral cavity in comparison with samples from the cervix uteri. STUDY DESIGN: In the present study, we used two well-established sets of PCR primers in different combinations for the detection of HPV DNA in 106 non-invasive brush biopsy samples of the oral mucosa and 56 samples from the cervix uteri. Direct sequencing of PCR products in all cases determined HPV genotype and specificity. RESULTS: Overall, HPV was detected in 69 of 106 oral mucosa samples. HPV specific amplicons were obtained in 35.8% (N = 38) when using GP5+/6+ primers. The positivity rate was increased to 65.1% in a GP5+/6+ auto-nested PCR approach. In contrast, MY9/11 PCR and nested PCR with MY9/11 outer followed by GP5+/6+ inner primers yielded 2.2% and 16.1%, respectively. In gynaecological samples, PCR results were similar independent of the primer combination used. Thus, DNA quality and DNA content could be additional factors influencing the rate of positivity. CONCLUSION: For oral mucosa samples, auto-nested GP5+/6+ PCR is in our hands the most suitable approach for epidemiological studies because of its high sensitivity, high reliability and reproducibility as well as its relatively simple laboratory procedure.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier Science
dc.publisher.placeNetherlands
dc.publisher.urihttp://www.elsevier.com/locate/jcv
dc.relation.ispartofpagefrom302
dc.relation.ispartofpageto308
dc.relation.ispartofissue4
dc.relation.ispartofjournalJournal of Clinical Virology
dc.relation.ispartofvolume30
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchMedical Microbiology
dc.subject.fieldofresearchcode0605
dc.subject.fieldofresearchcode1103
dc.subject.fieldofresearchcode1108
dc.titlePCR detection of human papillomavirus of the mucosa: comparison between MY09/11 and GP5+/6+ primer sets
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2004
gro.hasfulltextNo Full Text
gro.griffith.authorRemmerbach, Torsten W.


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