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  • Genomic microarray analysis

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    HamletPUB5.pdf (886.3Kb)
    File version
    Accepted Manuscript (AM)
    Author(s)
    Hamlet, S
    Petcu, E
    Ivanovski, S
    Griffith University Author(s)
    Hamlet, Stephen
    Petcu, Eugen B.
    Ivanovski, Saso
    Year published
    2015
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    Abstract
    Microarrays utilize classical hybridization techniques to measure the levels of expression of genomic sequences. Advances in microarray fabrication using robotic technology and bioinformatics, now means entire genomes can be interrogated with a high degree of sensitivity, specificity and throughput. To achieve this, RNA collected from samples is first transcribed into complimentary RNA (cRNA) that is subsequently fragmented and labeled with biotin. These fragments are allowed to hybridize with their complementary oligonucleotide sequence located in microscopic features on the array support media. Each of these features ...
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    Microarrays utilize classical hybridization techniques to measure the levels of expression of genomic sequences. Advances in microarray fabrication using robotic technology and bioinformatics, now means entire genomes can be interrogated with a high degree of sensitivity, specificity and throughput. To achieve this, RNA collected from samples is first transcribed into complimentary RNA (cRNA) that is subsequently fragmented and labeled with biotin. These fragments are allowed to hybridize with their complementary oligonucleotide sequence located in microscopic features on the array support media. Each of these features consists of hundreds of short oligonucleotide probes that match the target mRNA sequence. This results in an increase in abundance of biotinylated cRNA molecules on the feature to which a fluorescent dye is subsequently bound. The fluorescence levels can then be used to infer the relative abundance of specific mRNA sequences in the original sample. The microarray analysis of gene expression is thus a key weapon in the molecular biology arsenal that can open windows to the molecular processes manifested by the phenotypic differences observed between normal and diseased states.
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    Book Title
    Handbook of Vascular Biology Techniques
    DOI
    https://doi.org/10.1007/978-94-017-9716-0_30
    Copyright Statement
    © 2015 Springer. This is the author-manuscript version of this paper. It is reproduced here in accordance with the copyright policy of the publisher. Please refer to the publisher’s website for further information.
    Subject
    Periodontics
    Publication URI
    http://hdl.handle.net/10072/167372
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