dc.contributor.author | Yusof, AM | |
dc.contributor.author | Hu, NJ | |
dc.contributor.author | Wlodawer, A | |
dc.contributor.author | Hofmann, A | |
dc.date.accessioned | 2017-05-03T15:19:09Z | |
dc.date.available | 2017-05-03T15:19:09Z | |
dc.date.issued | 2005 | |
dc.date.modified | 2008-02-14T08:18:35Z | |
dc.identifier.issn | 0887-3585 | |
dc.identifier.uri | http://hdl.handle.net/10072/16740 | |
dc.description.abstract | Cyclase-associated protein (CAP) is a highly conserved and widely distributed protein that links the nutritional response signaling to cytoskeleton remodeling. In yeast, CAP is a component of the adenylyl cyclase complex and helps to activate the Ras-mediated catalytic cycle of the cyclase. While the N-terminal domain of CAP (NCAP) provides a binding site for adenylyl cyclase, the C-terminal domain (C-CAP) possesses actin binding activity. Our attempts to crystallize full-length recombinant CAP from Dictyostelium discoideum resulted in growth of orthorhombic crystals containing only the N-terminal domain (residues 42-227) due to auto-proteolytic cleavage. The structure was solved by molecular replacement with data at 2.2A͊resolution. The present crystal structure allows the characterization of a head-to-tail N-CAP dimer in the asymmetric unit and a crystallographic side-toside dimer. Comparison with previously published structures of N-CAP reveals variable modes of dimerization of this domain, but the presence of a common interface for the side-to-side dimer. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | WILEY-LISS, INC. | |
dc.publisher.place | United States | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofpagefrom | 255 | |
dc.relation.ispartofpageto | 262 | |
dc.relation.ispartofjournal | Proteins: Structure, Function, and Bioinformatics | |
dc.relation.ispartofvolume | 58 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Mathematical sciences | |
dc.subject.fieldofresearch | Biological sciences | |
dc.subject.fieldofresearch | Information and computing sciences | |
dc.subject.fieldofresearchcode | 49 | |
dc.subject.fieldofresearchcode | 31 | |
dc.subject.fieldofresearchcode | 46 | |
dc.title | Structural Evidence for Variable Oligomerization of the N-Terminal Domain of Cyclase-Associated Protein (CAP) | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.date.issued | 2005 | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Hofmann, Andreas | |