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dc.contributor.authorHatzirodos, Nicholas
dc.contributor.authorHummitzsch, Katja
dc.contributor.authorIrving-Rodgers, Helen F
dc.contributor.authorRodgers, Raymond J
dc.date.accessioned2017-10-30T12:31:49Z
dc.date.available2017-10-30T12:31:49Z
dc.date.issued2015
dc.identifier.issn1932-6203
dc.identifier.doi10.1371/journal.pone.0119800
dc.identifier.urihttp://hdl.handle.net/10072/169835
dc.description.abstractIn studies using isolated ovarian granulosa and thecal cells it is important to assess the degree of cross contamination. Marker genes commonly used for granulosa cells include FSHR, CYP19A1 and AMH while CYP17A1 and INSL3 are used for thecal cells. To increase the number of marker genes available we compared expression microarray data from isolated theca interna with that from granulosa cells of bovine small (n = 10 for both theca and granulosa cells; 3-5 mm) and large (n = 4 for both theca and granulosa cells, > 9 mm) antral follicles. Validation was conducted by qRT-PCR analyses. Known markers such as CYP19A1, FSHR and NR5A2 and another 11 genes (LOC404103, MGARP, GLDC, CHST8, CSN2, GPX3, SLC35G1, CA8, CLGN, FAM78A, SLC16A3) were common to the lists of the 50 most up regulated genes in granulosa cells from both follicle sizes. The expression in theca interna was more consistent than in granulosa cells between the two follicle sizes. Many genes up regulated in theca interna were common to both sizes of follicles (MGP, DCN, ASPN, ALDH1A1, COL1A2, FN1, COL3A1, OGN, APOD, COL5A2, IGF2, NID1, LHFP, ACTA2, DUSP12, ACTG2, SPARCL1, FILIP1L, EGFLAM, ADAMDEC1, HPGD, COL12A1, FBLN5, RAMP2, COL15A1, PLK2, COL6A3, LOXL1, RARRES1, FLI1, LAMA2). Many of these were stromal extracellular matrix genes. MGARP, GLDC, CHST8, GPX3 were identified as new potential markers for granulosa cells, while FBLN5, OGN, RAMP2 were significantly elevated in the theca interna.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherPublic Library of Science
dc.publisher.placeUnited States
dc.relation.ispartofpagefrome0119800-1
dc.relation.ispartofpagetoe0119800-13
dc.relation.ispartofissue3
dc.relation.ispartofjournalPloS One
dc.relation.ispartofvolume10
dc.subject.fieldofresearchAnimal Physiology - Cell
dc.subject.fieldofresearchcode060602
dc.titleTranscriptome comparisons identify new cell markers for theca interna and granulosa cells from small and large antral ovarian follicles
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2015 Hatzirodos et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
gro.hasfulltextFull Text
gro.griffith.authorIrving-Rodgers, Helen F.


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