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dc.contributor.authorSimmonds, Michael J
dc.contributor.authorMeiselman, Herbert J
dc.contributor.authorMarshall-Gradisnik, Sonya M
dc.contributor.authorPyne, Michael
dc.contributor.authorKakanis, Michael
dc.contributor.authorKeane, James
dc.contributor.authorBrenu, Ekua
dc.contributor.authorChristy, Rhys
dc.contributor.authorBaskurt, Oguz K
dc.description.abstractThe present study was designed to investigate the oxidant susceptibility of red blood cells (RBC) from four species (echidna, human, koala, Tasmanian devil) based on changes in cellular deformability. These species were specifically chosen based on differences in lifestyle and/or biology associated with varied levels of oxidative stress. The major focus was the influence of superoxide radicals generated within the cell (phenazine methosulfate, PMS, 50 μM) or in the extracellular medium (xanthine oxidase–hypoxanthine, XO–HX, 0.1 U/ml XO) on RBC deformability at various shear stresses (SS). RBC deformability was assessed by laser-diffraction analysis using a “slit-flow ektacytometer”. Both superoxide-generating treatments resulted in significant increases of methemoglobin for all species (p<0.01), with Tasmanian devil RBC demonstrating the most sensitivity to either treatment. PMS caused impaired RBC deformability for all species, but vast interspecies variations were observed: human and koala cells exhibited a similar sigmoid-like response to SS, short-beaked echidna values were markedly lower and only increased slightly with SS, while Tasmanian devil RBC were extremely rigid. The effect of XO–HX on RBC deformability was less when compared with PMS (i.e., smaller increase in rigidity) with the exception of Tasmanian devil RBC which exhibited essentially no deformation even at the highest SS; Tasmanian devil RBC response to XO–HX was thus comparable to that observed with PMS. Our findings indicate that ektacytometry can be used to determine the oxidant susceptibility of RBC from different species which varies significantly among mammals representing diverse lifestyles and evolutionary histories. These differences in susceptibility are consistent with species-specific discrepancies between observed and allometrically-predicted life spans and are compatible with the oxidant theory of aging.
dc.publisherI O S Press
dc.subject.fieldofresearchZoology not elsewhere classified
dc.subject.fieldofresearchBiomedical engineering
dc.subject.fieldofresearchClinical sciences
dc.titleAssessment of oxidant susceptibility of red blood cells in various species based on cell deformability
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorMarshall-Gradisnik, Sonya M.
gro.griffith.authorBrenu, Ekua
gro.griffith.authorSimmonds, Michael J.

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