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dc.contributor.authorZhou, Xiao-Ming
dc.contributor.authorEntwistle, Aiman
dc.contributor.authorZhang, Hong
dc.contributor.authorJackson, Antony P
dc.contributor.authorMason, Thomas O
dc.contributor.authorShimanovich, Ulyana
dc.contributor.authorKnowles, Tuomas PJ
dc.contributor.authorSmith, Andrew T
dc.contributor.authorSawyer, Elizabeth B
dc.contributor.authorPerrett, Sarah
dc.date.accessioned2018-01-23T00:44:52Z
dc.date.available2018-01-23T00:44:52Z
dc.date.issued2014
dc.identifier.issn1867-3880
dc.identifier.doi10.1002/cctc.201402125
dc.identifier.urihttp://hdl.handle.net/10072/171970
dc.description.abstractEnzyme immobilization is an important strategy to enhance the stability and recoverability of enzymes and to facilitate the separation of enzymes from reaction products. However, enzyme purification followed by separate chemical steps to allow immobilization on a solid support reduces the efficiency and yield of the active enzyme. Here we describe polypeptide constructs that self-assemble spontaneously into nanofibrils with fused active enzyme subunits displayed on the amyloid fibril surface. We measured the steady-state kinetic parameters for the appended enzymes in situ within fibrils and compare these with the identical protein constructs in solution. Finally, we demonstrated that the fibrils can be recycled and reused in functional assays both in conventional batch processes and in a continuous-flow microreactor.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherWiley- VCH Verlag GmbH & Co. KGaA
dc.relation.ispartofpagefrom1961
dc.relation.ispartofpageto1968
dc.relation.ispartofissue7
dc.relation.ispartofjournalChemCatChem
dc.relation.ispartofvolume6
dc.subject.fieldofresearchInorganic Chemistry not elsewhere classified
dc.subject.fieldofresearchInorganic Chemistry
dc.subject.fieldofresearchPhysical Chemistry (incl. Structural)
dc.subject.fieldofresearchChemical Engineering
dc.subject.fieldofresearchcode030299
dc.subject.fieldofresearchcode0302
dc.subject.fieldofresearchcode0306
dc.subject.fieldofresearchcode0904
dc.titleSelf-assembly of amyloid fibrils that display active enzymes
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.description.versionPublished
gro.rights.copyright© 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
gro.hasfulltextFull Text
gro.griffith.authorSmith, Andrew T.


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