Show simple item record

dc.contributor.authorValentini, Alice
dc.contributor.authorTaberlet, Pierre
dc.contributor.authorMiaud, Claude
dc.contributor.authorCivade, Raphael
dc.contributor.authorHerder, Jelger
dc.contributor.authorThomsen, Philip Francis
dc.contributor.authorBellemain, Eva
dc.contributor.authorBesnard, Aurelien
dc.contributor.authorCoissac, Eric
dc.contributor.authorBoyer, Frederic
dc.contributor.authorGaboriaud, Coline
dc.contributor.authorJean, Pauline
dc.contributor.authorPoulet, Nicolas
dc.contributor.authorRoset, Nicolas
dc.contributor.authorCopp, Gordon H.
dc.contributor.authorGeniez, Philippe
dc.contributor.authorPont, Didier
dc.contributor.authorArgillier, Christine
dc.contributor.authorBaudoin, Jean-Marc
dc.contributor.authorPeroux, Tiphaine
dc.contributor.authorCrivelli, Alain J.
dc.contributor.authorOlivier, Anthony
dc.contributor.authorAcqueberge, Manon
dc.contributor.authorLe Brun, Matthieu
dc.contributor.authorMoller, Peter Rask
dc.contributor.authorWillerslev, Eske
dc.contributor.authorDejean, Tony
dc.date.accessioned2017-11-26T23:57:50Z
dc.date.available2017-11-26T23:57:50Z
dc.date.issued2016
dc.identifier.issn1365-294X
dc.identifier.doi10.1111/mec.13428
dc.identifier.urihttp://hdl.handle.net/10072/172157
dc.description.abstractGlobal biodiversity in freshwater and the oceans is declining at high rates. Reliable tools for assessing and monitoring aquatic biodiversity, especially for rare and secretive species, are important for efficient and timely management. Recent advances in DNA sequencing have provided a new tool for species detection from DNA present in the environment. In this study, we tested whether an environmental DNA (eDNA) metabarcoding approach, using water samples, can be used for addressing significant questions in ecology and conservation. Two key aquatic vertebrate groups were targeted: amphibians and bony fish. The reliability of this method was cautiously validated in silico, in vitro and in situ. When compared with traditional surveys or historical data, eDNA metabarcoding showed a much better detection probability overall. For amphibians, the detection probability with eDNA metabarcoding was 0.97 (CI = 0.90–0.99) vs. 0.58 (CI = 0.50–0.63) for traditional surveys. For fish, in 89% of the studied sites, the number of taxa detected using the eDNA metabarcoding approach was higher or identical to the number detected using traditional methods. We argue that the proposed DNA-based approach has the potential to become the next-generation tool for ecological studies and standardized biodiversity monitoring in a wide range of aquatic ecosystems.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherBlackwell Publishing Ltd
dc.relation.ispartofpagefrom929
dc.relation.ispartofpageto942
dc.relation.ispartofissue4
dc.relation.ispartofjournalMolecular Ecology
dc.relation.ispartofvolume25
dc.subject.fieldofresearchPopulation, Ecological and Evolutionary Genetics
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchcode060411
dc.subject.fieldofresearchcode06
dc.titleNext-generation monitoring of aquatic biodiversity using environmental DNA metabarcoding
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorWillerslev, Eske


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record