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dc.contributor.authorVinner, Lasse
dc.contributor.authorMourier, Tobias
dc.contributor.authorFriis-Nielsen, Jens
dc.contributor.authorGniadecki, Robert
dc.contributor.authorDybkaer, Karen
dc.contributor.authorRosenberg, Jacob
dc.contributor.authorLanghoff, Jill Levin
dc.contributor.authorCruz, David Flores Santa
dc.contributor.authorFonager, Jannik
dc.contributor.authorIzarzugaza, Jose M. G.
dc.contributor.authorGupta, Ramneek
dc.contributor.authorSicheritz-Ponten, Thomas
dc.contributor.authorBrunak, Soren
dc.contributor.authorWillerslev, Eske
dc.contributor.authorNielsen, Lars Peter
dc.contributor.authorHansen, Anders Johannes
dc.date.accessioned2017-12-04T06:14:29Z
dc.date.available2017-12-04T06:14:29Z
dc.date.issued2015
dc.identifier.issn2045-2322
dc.identifier.doi10.1038/srep13201
dc.identifier.urihttp://hdl.handle.net/10072/172167
dc.description.abstractAlthough nearly one fifth of all human cancers have an infectious aetiology, the causes for the majority of cancers remain unexplained. Despite the enormous data output from high-throughput shotgun sequencing, viral DNA in a clinical sample typically constitutes a proportion of host DNA that is too small to be detected. Sequence variation among virus genomes complicates application of sequence-specific, and highly sensitive, PCR methods. Therefore, we aimed to develop and characterize a method that permits sensitive detection of sequences despite considerable variation. We demonstrate that our low-stringency in-solution hybridization method enables detection of <100 viral copies. Furthermore, distantly related proviral sequences may be enriched by orders of magnitude, enabling discovery of hitherto unknown viral sequences by high-throughput sequencing. The sensitivity was sufficient to detect retroviral sequences in clinical samples. We used this method to conduct an investigation for novel retrovirus in samples from three cancer types. In accordance with recent studies our investigation revealed no retroviral infections in human B-cell lymphoma cells, cutaneous T-cell lymphoma or colorectal cancer biopsies. Nonetheless, our generally applicable method makes sensitive detection possible and permits sequencing of distantly related sequences from complex material.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Macmillan
dc.relation.ispartofpagefrom13201-1
dc.relation.ispartofpageto13201-13
dc.relation.ispartofjournalScientific Reports
dc.relation.ispartofvolume5
dc.subject.fieldofresearchBiochemistry and Cell Biology not elsewhere classified
dc.subject.fieldofresearchcode060199
dc.titleInvestigation of Human Cancers for Retrovirus by Low-Stringency Target Enrichment and High-Throughput Sequencing
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2015. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
gro.hasfulltextFull Text
gro.griffith.authorWillerslev, Eske


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