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dc.contributor.authorSeguin-Orlando, Andaine
dc.contributor.authorGamba, Cristina
dc.contributor.authorDer Sarkissian, Clio
dc.contributor.authorErmini, Luca
dc.contributor.authorLouvel, Guillaume
dc.contributor.authorBoulygina, Eugenia
dc.contributor.authorSokolov, Alexey
dc.contributor.authorNedoluzhko, Artem
dc.contributor.authorLorenzen, Eline D.
dc.contributor.authorLopez, Patricio
dc.contributor.authorMcDonald, H. Gregory
dc.contributor.authorScott, Eric
dc.contributor.authorTikhonov, Alexei
dc.contributor.authorStafford Jr, Thomas W.
dc.contributor.authorAlfarhan, Ahmed H.
dc.contributor.authorAlquraishi, Saleh A.
dc.contributor.authorAl-Rasheid, Khaled A. S.
dc.contributor.authorShapiro, Beth
dc.contributor.authorWillerslev, Eske
dc.contributor.authorProkhortchouk, Egor
dc.contributor.authorOrlando, Ludovic
dc.date.accessioned2017-12-04T05:58:20Z
dc.date.available2017-12-04T05:58:20Z
dc.date.issued2015
dc.identifier.issn2045-2322en_US
dc.identifier.doi10.1038/srep11826en_US
dc.identifier.urihttp://hdl.handle.net/10072/172168
dc.description.abstractThe recent discovery that DNA methylation survives in fossil material provides an opportunity for novel molecular approaches in palaeogenomics. Here, we apply to ancient DNA extracts the probe-independent Methylated Binding Domains (MBD)-based enrichment method, which targets DNA molecules containing methylated CpGs. Using remains of a Palaeo-Eskimo Saqqaq individual, woolly mammoths, polar bears and two equine species, we confirm that DNA methylation survives in a variety of tissues, environmental contexts and over a large temporal range (4,000 to over 45,000 years before present). MBD enrichment, however, appears principally biased towards the recovery of CpG-rich and long DNA templates and is limited by the fast post-mortem cytosine deamination rates of methylated epialleles. This method, thus, appears only appropriate for the analysis of ancient methylomes from very well preserved samples, where both DNA fragmentation and deamination have been limited. This work represents an essential step toward the characterization of ancient methylation signatures, which will help understanding the role of epigenetic changes in past environmental and cultural transitions.en_US
dc.description.peerreviewedYesen_US
dc.languageEnglishen_US
dc.publisherNature Macmillanen_US
dc.relation.ispartofpagefrom11826-1en_US
dc.relation.ispartofpageto11826-15en_US
dc.relation.ispartofjournalScientific Reportsen_US
dc.relation.ispartofvolume5en_US
dc.subject.fieldofresearchBiochemistry and Cell Biology not elsewhere classifieden_US
dc.subject.fieldofresearchcode060199en_US
dc.titlePros and cons of methylation-based enrichment methods for ancient DNAen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/en_US
dc.description.versionPublisheden_US
gro.rights.copyright© The Author(s) 2015. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en_US
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gro.griffith.authorWillerslev, Eske


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