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dc.contributor.authorSeguin-Orlando, Andaine
dc.contributor.authorGamba, Cristina
dc.contributor.authorDer Sarkissian, Clio
dc.contributor.authorErmini, Luca
dc.contributor.authorLouvel, Guillaume
dc.contributor.authorBoulygina, Eugenia
dc.contributor.authorSokolov, Alexey
dc.contributor.authorNedoluzhko, Artem
dc.contributor.authorLorenzen, Eline D.
dc.contributor.authorLopez, Patricio
dc.contributor.authorMcDonald, H. Gregory
dc.contributor.authorScott, Eric
dc.contributor.authorTikhonov, Alexei
dc.contributor.authorStafford Jr, Thomas W.
dc.contributor.authorAlfarhan, Ahmed H.
dc.contributor.authorAlquraishi, Saleh A.
dc.contributor.authorAl-Rasheid, Khaled A. S.
dc.contributor.authorShapiro, Beth
dc.contributor.authorWillerslev, Eske
dc.contributor.authorProkhortchouk, Egor
dc.contributor.authorOrlando, Ludovic
dc.date.accessioned2017-12-04T05:58:20Z
dc.date.available2017-12-04T05:58:20Z
dc.date.issued2015
dc.identifier.issn2045-2322
dc.identifier.doi10.1038/srep11826
dc.identifier.urihttp://hdl.handle.net/10072/172168
dc.description.abstractThe recent discovery that DNA methylation survives in fossil material provides an opportunity for novel molecular approaches in palaeogenomics. Here, we apply to ancient DNA extracts the probe-independent Methylated Binding Domains (MBD)-based enrichment method, which targets DNA molecules containing methylated CpGs. Using remains of a Palaeo-Eskimo Saqqaq individual, woolly mammoths, polar bears and two equine species, we confirm that DNA methylation survives in a variety of tissues, environmental contexts and over a large temporal range (4,000 to over 45,000 years before present). MBD enrichment, however, appears principally biased towards the recovery of CpG-rich and long DNA templates and is limited by the fast post-mortem cytosine deamination rates of methylated epialleles. This method, thus, appears only appropriate for the analysis of ancient methylomes from very well preserved samples, where both DNA fragmentation and deamination have been limited. This work represents an essential step toward the characterization of ancient methylation signatures, which will help understanding the role of epigenetic changes in past environmental and cultural transitions.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Macmillan
dc.relation.ispartofpagefrom11826-1
dc.relation.ispartofpageto11826-15
dc.relation.ispartofjournalScientific Reports
dc.relation.ispartofvolume5
dc.subject.fieldofresearchBiochemistry and Cell Biology not elsewhere classified
dc.subject.fieldofresearchcode060199
dc.titlePros and cons of methylation-based enrichment methods for ancient DNA
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2015. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
gro.hasfulltextFull Text
gro.griffith.authorWillerslev, Eske


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