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  • Electrochemical detection of protein glycosylation using lectin and protein-gold affinity interactions

    Author(s)
    Yadav, Sharda
    Carrascosa, Laura G
    Sina, Abu AI
    Shiddiky, Muhammad JA
    Hill, Michelle M
    Trau, Matt
    Griffith University Author(s)
    Shiddiky, Muhammad J.
    Yadav, Sharda
    Year published
    2016
    Metadata
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    Abstract
    We report a new method for the electrochemical detection of glycosylation on proteins, which relies on lectin–protein interaction on a bare gold electrode. The target protein isolated by immunoaffinity is directly adsorbed onto a gold surface and its glycosylation status is retrieved by subsequent addition of specific lectins. The adsorption and subsequent recognition process is monitored electrochemically in the presence of [Fe(CN)6]3−/4− redox system. By decoupling target protein capture from glycosylation read-out steps, this approach circumvents unwanted antibody–lectin crosstalk while enabling specific glycosylation ...
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    We report a new method for the electrochemical detection of glycosylation on proteins, which relies on lectin–protein interaction on a bare gold electrode. The target protein isolated by immunoaffinity is directly adsorbed onto a gold surface and its glycosylation status is retrieved by subsequent addition of specific lectins. The adsorption and subsequent recognition process is monitored electrochemically in the presence of [Fe(CN)6]3−/4− redox system. By decoupling target protein capture from glycosylation read-out steps, this approach circumvents unwanted antibody–lectin crosstalk while enabling specific glycosylation detection of a glycoprotein in serum-spiked samples in less than 1 h.
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    Journal Title
    Analyst
    Volume
    141
    Issue
    8
    DOI
    https://doi.org/10.1039/c6an00528d
    Subject
    Analytical chemistry
    Analytical chemistry not elsewhere classified
    Other chemical sciences
    Publication URI
    http://hdl.handle.net/10072/172418
    Collection
    • Journal articles

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