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dc.contributor.authorYadav, Sharda
dc.contributor.authorCarrascosa, Laura G
dc.contributor.authorSina, Abu AI
dc.contributor.authorShiddiky, Muhammad JA
dc.contributor.authorHill, Michelle M
dc.contributor.authorTrau, Matt
dc.date.accessioned2018-03-08T04:42:32Z
dc.date.available2018-03-08T04:42:32Z
dc.date.issued2016
dc.identifier.issn0003-2654
dc.identifier.doi10.1039/c6an00528d
dc.identifier.urihttp://hdl.handle.net/10072/172418
dc.description.abstractWe report a new method for the electrochemical detection of glycosylation on proteins, which relies on lectin–protein interaction on a bare gold electrode. The target protein isolated by immunoaffinity is directly adsorbed onto a gold surface and its glycosylation status is retrieved by subsequent addition of specific lectins. The adsorption and subsequent recognition process is monitored electrochemically in the presence of [Fe(CN)6]3−/4− redox system. By decoupling target protein capture from glycosylation read-out steps, this approach circumvents unwanted antibody–lectin crosstalk while enabling specific glycosylation detection of a glycoprotein in serum-spiked samples in less than 1 h.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherR S C Publications
dc.relation.ispartofpagefrom2356
dc.relation.ispartofpageto2361
dc.relation.ispartofissue8
dc.relation.ispartofjournalAnalyst
dc.relation.ispartofvolume141
dc.subject.fieldofresearchAnalytical Chemistry not elsewhere classified
dc.subject.fieldofresearchAnalytical Chemistry
dc.subject.fieldofresearchOther Chemical Sciences
dc.subject.fieldofresearchcode030199
dc.subject.fieldofresearchcode0301
dc.subject.fieldofresearchcode0399
dc.titleElectrochemical detection of protein glycosylation using lectin and protein-gold affinity interactions
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorShiddiky, Muhammad J.
gro.griffith.authorYadav, Sharda


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