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dc.contributor.authorTsao, Simon Chang-Hao
dc.contributor.authorVaidyanathan, Ramanathan
dc.contributor.authorDey, Shuvashis
dc.contributor.authorCarrascosa, Laura G
dc.contributor.authorChristophi, Christopher
dc.contributor.authorCebon, Jonathan
dc.contributor.authorShiddiky, Muhammad JA
dc.contributor.authorBehren, Andreas
dc.contributor.authorTrau, Matt
dc.date.accessioned2019-01-18T12:32:36Z
dc.date.available2019-01-18T12:32:36Z
dc.date.issued2016
dc.identifier.issn2045-2322
dc.identifier.doi10.1038/srep19709
dc.identifier.urihttp://hdl.handle.net/10072/172420
dc.description.abstractWith new systemic therapies becoming available for metastatic melanoma such as BRAF and PD-1 inhibitors, there is an increasing demand for methods to assist with treatment selection and response monitoring. Quantification and characterisation of circulating melanoma cells (CMCs) has been regarded as an excellent non-invasive candidate but a sensitive and efficient tool to do these is lacking. Herein we demonstrate a microfluidic approach for melanoma cell capture and subsequent on-chip evaluation of BRAF mutation status. Our approach utilizes a recently discovered alternating current electrohydrodynamic (AC-EHD)-induced surface shear forces, referred to as nanoshearing. A key feature of nanoshearing is the ability to agitate fluid to encourage contact with surface-bound antibody for the cell capture whilst removing nonspecific cells from the surface. By adjusting the AC-EHD force to match the binding affinity of antibodies against the melanoma-associated chondroitin sulphate proteoglycan (MCSP), a commonly expressed melanoma antigen, this platform achieved an average recovery of 84.7% from biological samples. Subsequent staining with anti-BRAFV600E specific antibody enabled on-chip evaluation of BRAFV600E mutation status in melanoma cells. We believe that the ability of nanoshearing-based capture to enumerate melanoma cells and subsequent on-chip characterisation has the potential as a rapid screening tool while making treatment decisions.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofpagefrom19709-1
dc.relation.ispartofpageto19709-10
dc.relation.ispartofjournalScientific Reports
dc.relation.ispartofvolume6
dc.subject.fieldofresearchBiochemistry and Cell Biology not elsewhere classified
dc.subject.fieldofresearchcode060199
dc.titleCapture and On-chip analysis of Melanoma Cells Using Tunable Surface Shear forces
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.
gro.hasfulltextFull Text
gro.griffith.authorShiddiky, Muhammad J.


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