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dc.contributor.authorYadav, Sharda
dc.contributor.authorBoriachek, Kseniia
dc.contributor.authorIslam, Md Nazmul
dc.contributor.authorLobb, Richard
dc.contributor.authorMoller, Andreas
dc.contributor.authorHill, Michelle M
dc.contributor.authorAl Hossain, Md Shahriar
dc.contributor.authorNam-Trung, Nguyen
dc.contributor.authorShiddiky, Muhammad JA
dc.date.accessioned2018-03-08T04:56:35Z
dc.date.available2018-03-08T04:56:35Z
dc.date.issued2017
dc.identifier.issn2196-0216
dc.identifier.doi10.1002/celc.201600391
dc.identifier.urihttp://hdl.handle.net/10072/172421
dc.description.abstractExosomes are cell-derived vesicles secreted by both normal and cancerous cells into the extracellular matrix and in blood circulation. Tumor-derived exosomes have attracted increasing attention in noninvasive cancer diagnosis and prognosis. However, their effective capture and specific detection pose significant technical challenges. Current detection methods largely fail to quantify the tumor-derived exosomes present in the total (bulk) exosome population derived from body fluids of cancer patients. In this proof-of-concept study, we report an electrochemical detection method to directly quantify the disease-specific exosomes present in cell culture media. The assay has a two-step design, where bulk exosome populations are initially captured by using a generic antibody (i.e. tetraspanin biomarker, CD9). Subsequent detection of the cancer-specific exosomes within the captured exosomes was carried out by using a cancer-specific antibody, in this case, a human epidermal growth factor receptor 2 (HER-2) antibody, allowing quantification of HER2-postive, breast-cancer-derived exosomes. This approach exhibits excellent specificity for HER-2(+) BT-474 cell-derived exosomes (detection limit, 4.7×105 exosomes μL−1) with a relative standard deviation of <4.9 % (n=3). We suggest that this simple and inexpensive electrochemical method could be an alternative for the quantification of exosome subpopulations in specific disease settings for future clinical bioassays.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto6
dc.relation.ispartofjournalChemElectroChem
dc.relation.ispartofvolume3
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchAnalytical chemistry not elsewhere classified
dc.subject.fieldofresearchPhysical chemistry
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchMacromolecular and materials chemistry
dc.subject.fieldofresearchOrganic chemistry
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode340199
dc.subject.fieldofresearchcode3406
dc.subject.fieldofresearchcode3499
dc.subject.fieldofresearchcode3403
dc.subject.fieldofresearchcode3405
dc.titleAn Electrochemical Method for the Detection of Disease-Specific Exosomes
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Sciences, School of Natural Sciences
gro.hasfulltextNo Full Text
gro.griffith.authorNguyen, Nam-Trung
gro.griffith.authorShiddiky, Muhammad J.
gro.griffith.authorYadav, Sharda


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