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dc.contributor.authorGrewal, Yadveer S
dc.contributor.authorShiddiky, Muhammad JA
dc.contributor.authorSpadafora, Lauren J
dc.contributor.authorCangelosi, Gerard A
dc.contributor.authorTrau, Matt
dc.date.accessioned2018-02-27T03:02:24Z
dc.date.available2018-02-27T03:02:24Z
dc.date.issued2015
dc.identifier.issn1932-7447
dc.identifier.doi10.1021/acs.jpcc.5b01234
dc.identifier.urihttp://hdl.handle.net/10072/172426
dc.description.abstractNanoyeast single-chain variable fragments (nanoyeast-scFv) are a new class of low-cost and stable protein capture agents developed as alternatives to full length monoclonal antibodies for use in immunosensors. Physical characteristics that impart nanoyeast-scFv with these advantages have yet to be investigated. We investigate the structure, size and surface loading of nanoyeast-scFv to better understand its ability to specifically and sensitively capture proteins of interest while retaining activity in solution. Nanoyeast-scFv fragments were found to be globular in structure and heterogeneous in size, typically <100 nm, and were found to aggregate in solution. Optimal fragment size was determined to be <220 nm, with larger fragments interfering with the electrochemical biosensor. We hypothesize that the stability of nanoyeast-scFv is conferred in part by components of the yeast cell wall fragments, which provide stability to the associated scFv both during selection, lyophilization, and rehydration.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.relation.ispartofpagefrom12674
dc.relation.ispartofpageto12680
dc.relation.ispartofissue22
dc.relation.ispartofjournalJournal of Physical Chemistry C
dc.relation.ispartofvolume119
dc.subject.fieldofresearchChemical sciences
dc.subject.fieldofresearchEngineering
dc.subject.fieldofresearchBiomedical engineering not elsewhere classified
dc.subject.fieldofresearchcode34
dc.subject.fieldofresearchcode40
dc.subject.fieldofresearchcode400399
dc.titleStructural Characterization of Nanoyeast Single-Chain Fragment Variable Affinity Reagents
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorShiddiky, Muhammad J.


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