dc.contributor.author | Grewal, Yadveer S | |
dc.contributor.author | Shiddiky, Muhammad JA | |
dc.contributor.author | Spadafora, Lauren J | |
dc.contributor.author | Cangelosi, Gerard A | |
dc.contributor.author | Trau, Matt | |
dc.date.accessioned | 2018-02-27T03:02:24Z | |
dc.date.available | 2018-02-27T03:02:24Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1932-7447 | |
dc.identifier.doi | 10.1021/acs.jpcc.5b01234 | |
dc.identifier.uri | http://hdl.handle.net/10072/172426 | |
dc.description.abstract | Nanoyeast single-chain variable fragments (nanoyeast-scFv) are a new class of low-cost and stable protein capture agents developed as alternatives to full length monoclonal antibodies for use in immunosensors. Physical characteristics that impart nanoyeast-scFv with these advantages have yet to be investigated. We investigate the structure, size and surface loading of nanoyeast-scFv to better understand its ability to specifically and sensitively capture proteins of interest while retaining activity in solution. Nanoyeast-scFv fragments were found to be globular in structure and heterogeneous in size, typically <100 nm, and were found to aggregate in solution. Optimal fragment size was determined to be <220 nm, with larger fragments interfering with the electrochemical biosensor. We hypothesize that the stability of nanoyeast-scFv is conferred in part by components of the yeast cell wall fragments, which provide stability to the associated scFv both during selection, lyophilization, and rehydration. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | American Chemical Society | |
dc.relation.ispartofpagefrom | 12674 | |
dc.relation.ispartofpageto | 12680 | |
dc.relation.ispartofissue | 22 | |
dc.relation.ispartofjournal | Journal of Physical Chemistry C | |
dc.relation.ispartofvolume | 119 | |
dc.subject.fieldofresearch | Chemical sciences | |
dc.subject.fieldofresearch | Engineering | |
dc.subject.fieldofresearch | Biomedical engineering not elsewhere classified | |
dc.subject.fieldofresearchcode | 34 | |
dc.subject.fieldofresearchcode | 40 | |
dc.subject.fieldofresearchcode | 400399 | |
dc.title | Structural Characterization of Nanoyeast Single-Chain Fragment Variable Affinity Reagents | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Shiddiky, Muhammad J. | |