dc.contributor.author | Vaidyanathan, Ramanathan | |
dc.contributor.author | van Leeuwen, Lara Michelle | |
dc.contributor.author | Rauf, Sakandar | |
dc.contributor.author | Shiddiky, Muhammad JA | |
dc.contributor.author | Trau, Matt | |
dc.date.accessioned | 2019-01-18T12:32:37Z | |
dc.date.available | 2019-01-18T12:32:37Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.doi | 10.1038/srep09756 | |
dc.identifier.uri | http://hdl.handle.net/10072/172427 | |
dc.description.abstract | Microfluidic flow based multiplexed devices have gained significant promise in detecting biomarkers in
complex biological samples. However, to fully exploit their use in bioanalysis, issues such as (i) low
sensitivity and (ii) high levels of nonspecific adsorption of non-target species have to be overcome. Herein,
we describe a new multiplexed device for the sensitive detection of multiple protein biomarkers in serum by
using an alternating current (ac) electrohydrodynamics (ac-EHD) induced surface shear forces based
phenomenon referred to as nanoshearing. The tunable nature (via manipulation of ac field) of these
nanoshearing forces can alter the capture performance of the device (e.g., improved fluid transport enhances
number of sensor-target collisions). This can also selectively displace weakly (nonspecifically) bound
molecules from the electrode surface (i.e., fluid shear forces can be tuned to shear away nonspecific species
present in biological samples). Using this approach, we achieved sensitive (100 fg mL21) naked eye detection
of multiple protein targets spiked in human serum and a 1000-fold enhancement in comparison to
hydrodynamic flow based devices for biomarker detection. We believe that this approach could potentially
represent a clinical diagnostic tool that can be integrated into resource-limited settings for sensitive
detection of target biomarkers using naked eye. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Nature Publishing Group | |
dc.relation.ispartofpagefrom | 9756-1 | |
dc.relation.ispartofpageto | 9756-7 | |
dc.relation.ispartofjournal | Scientific Reports | |
dc.relation.ispartofvolume | 5 | |
dc.subject.fieldofresearch | Biomedical engineering not elsewhere classified | |
dc.subject.fieldofresearchcode | 400399 | |
dc.title | A Multiplexed Device Based on Tunable Nanoshearing for Specific Detection of Multiple Protein Biomarkers in Serum | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
dcterms.license | http://creativecommons.org/licenses/by/4.0/ | |
dc.description.version | Version of Record (VoR) | |
gro.rights.copyright | © The Author(s). 2015. This work is licensed under a Creative Commons Attribution 4.0 International
License. The images or other third party material in this article are included in the
article’s Creative Commons license, unless indicated otherwise in the credit line; if
the material is not included under the Creative Commons license, users will need
to obtain permission fromthe license holder in order to reproduce thematerial. To
view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Shiddiky, Muhammad J. | |