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dc.contributor.authorVaidyanathan, Ramanathan
dc.contributor.authorNaghibosadat, Maedeh
dc.contributor.authorRauf, Sakandar
dc.contributor.authorKorbie, Darren
dc.contributor.authorCarrascosa, Laura G
dc.contributor.authorShiddiky, Muhammad JA
dc.contributor.authorTrau, Matt
dc.date.accessioned2018-02-27T02:35:58Z
dc.date.available2018-02-27T02:35:58Z
dc.date.issued2014
dc.identifier.issn0003-2700
dc.identifier.doi10.1021/ac502082b
dc.identifier.urihttp://hdl.handle.net/10072/172431
dc.description.abstractExosomes show promise as noninvasive biomarkers for cancer, but their effective capture and specific detection is a significant challenge. Herein, we report a multiplexed microfluidic device for highly specific capture and detection of multiple exosome targets using a tunable alternating current electrohydrodynamic (ac-EHD) methodology, referred to as nanoshearing. In our system, electrical body forces generated by ac-EHD act within nanometers of an electrode surface (i.e., within the electrical layer) to generate nanoscaled fluid flow that enhances the specificity of capture and also reduce nonspecific adsorption of weakly bound molecules from the electrode surface. This approach demonstrates the analysis of exosomes derived from cells expressing human epidermal growth factor receptor 2 (HER2) and prostate specific antigen (PSA), and is also capable of specifically isolating exosomes from breast cancer patient samples. The device also exhibited a 3-fold enhancement in detection sensitivity in comparison to hydrodynamic flow based assays (LOD 2760 exosomes/μL for ac-EHD vs LOD 8300 exosomes/μL for hydrodynamic flow; (n = 3)). We propose this approach can potentially have relevance as a simple and rapid quantification tool to analyze exosome targets in biological applications.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.relation.ispartofpagefrom11125
dc.relation.ispartofpageto11132
dc.relation.ispartofissue22
dc.relation.ispartofjournalAnalytical Chemistry
dc.relation.ispartofvolume86
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchAnalytical chemistry not elsewhere classified
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode340199
dc.subject.fieldofresearchcode3499
dc.titleDetecting Exosomes Specifically: A Multiplexed Device Based on Alternating Current Electrohydrodynamic Induced Nanoshearing
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionAccepted Manuscript (AM)
gro.rights.copyrightThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright 2014 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/ac502082b
gro.hasfulltextFull Text
gro.griffith.authorShiddiky, Muhammad J.


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