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  • eMethylsorb: Electrochemical Quantification Of DNA Methylation at CPG Resolution Using DNA–Gold Affinity Interactions

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    Accepted Manuscript (AM)
    Author(s)
    Ibn Sina, Abu Ali
    Howell, Sidney
    Carrascosa, Laura G
    Rauf, Sakandar
    Shiddiky, Muhammad JA
    Trau, Matt
    Griffith University Author(s)
    Shiddiky, Muhammad J.
    Year published
    2014
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    Abstract
    We report a simple electrochemical method referred to as “eMethylsorb” for the detection of DNA methylation. The method relies on the base dependent affinity interaction of DNA with gold. The methylation status of DNA is quantified by monitoring the electrochemical current as a function of the relative adsorption level of bisulphite treated DNA samples onto a bare gold electrode. This method can successfully distinguish methylated and unmethylated epigenotypes at single CpG resolution.We report a simple electrochemical method referred to as “eMethylsorb” for the detection of DNA methylation. The method relies on the base dependent affinity interaction of DNA with gold. The methylation status of DNA is quantified by monitoring the electrochemical current as a function of the relative adsorption level of bisulphite treated DNA samples onto a bare gold electrode. This method can successfully distinguish methylated and unmethylated epigenotypes at single CpG resolution.
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    Journal Title
    Chemical Communications
    Volume
    50
    Issue
    86
    DOI
    https://doi.org/10.1039/c4cc06732k
    Copyright Statement
    © 2014 Royal Society of Chemistry. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
    Subject
    Chemical sciences
    Analytical chemistry not elsewhere classified
    Publication URI
    http://hdl.handle.net/10072/172433
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    • Journal articles

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