eMethylsorb: Electrochemical Quantification Of DNA Methylation at CPG Resolution Using DNA–Gold Affinity Interactions
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We report a simple electrochemical method referred to as “eMethylsorb” for the detection of DNA methylation. The method relies on the base dependent affinity interaction of DNA with gold. The methylation status of DNA is quantified by monitoring the electrochemical current as a function of the relative adsorption level of bisulphite treated DNA samples onto a bare gold electrode. This method can successfully distinguish methylated and unmethylated epigenotypes at single CpG resolution.
© 2014 Royal Society of Chemistry. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
Analytical Chemistry not elsewhere classified