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dc.contributor.authorIbn Sina, Abu Ali
dc.contributor.authorCarrascosa, Laura G
dc.contributor.authorPalanisamy, Ramkumar
dc.contributor.authorRauf, Sakandar
dc.contributor.authorShiddiky, Muhammad JA
dc.contributor.authorTrau, Matt
dc.date.accessioned2018-02-27T02:09:35Z
dc.date.available2018-02-27T02:09:35Z
dc.date.issued2014
dc.identifier.issn0003-2700
dc.identifier.doi10.1021/ac502214z
dc.identifier.urihttp://hdl.handle.net/10072/172434
dc.description.abstractThe analysis of DNA methylation is becoming increasingly important both in the clinic and also as a research tool to unravel key epigenetic molecular mechanisms in biology. Current methodologies for the quantification of regional DNA methylation (i.e., the average methylation over a region of DNA in the genome) are largely affected by comprehensive DNA sequencing methodologies which tend to be expensive, tedious, and time-consuming for many applications. Herein, we report an alternative DNA methylation detection method referred to as “Methylsorb”, which is based on the inherent affinity of DNA bases to the gold surface (i.e., the trend of the affinity interactions is adenine > cytosine ≥ guanine > thymine).1 Since the degree of gold–DNA affinity interaction is highly sequence dependent, it provides a new capability to detect DNA methylation by simply monitoring the relative adsorption of bisulfite treated DNA sequences onto a gold chip. Because the selective physical adsorption of DNA fragments to gold enable a direct read-out of regional DNA methylation, the current requirement for DNA sequencing is obviated. To demonstrate the utility of this method, we present data on the regional methylation status of two CpG clusters located in the EN1 and MIR200B genes in MCF7 and MDA-MB-231 cells. The methylation status of these regions was obtained from the change in relative mass on gold surface with respect to relative adsorption of an unmethylated DNA source and this was detected using surface plasmon resonance (SPR) in a label-free and real-time manner. We anticipate that the simplicity of this method, combined with the high level of accuracy for identifying the methylation status of cytosines in DNA, could find broad application in biology and diagnostics.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.relation.ispartofpagefrom10179
dc.relation.ispartofpageto10185
dc.relation.ispartofissue20
dc.relation.ispartofjournalAnalytical Chemistry
dc.relation.ispartofvolume86
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchAnalytical chemistry not elsewhere classified
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode340199
dc.subject.fieldofresearchcode3499
dc.titleMethylsorb: A Simple Method for Quantifying DNA Methylation Using DNA-Gold Affinity Interactions
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorShiddiky, Muhammad J.


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