dc.contributor.author | Wang, Yuling | |
dc.contributor.author | Rauf, Sakandar | |
dc.contributor.author | Grewal, Yadveer S | |
dc.contributor.author | Spadafora, Lauren J | |
dc.contributor.author | Shiddiky, Muhammad JA | |
dc.contributor.author | Cangelosi, Gerard A | |
dc.contributor.author | Schluecker, Sebastian | |
dc.contributor.author | Trau, Matt | |
dc.date.accessioned | 2018-07-27T01:30:33Z | |
dc.date.available | 2018-07-27T01:30:33Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0003-2700 | |
dc.identifier.doi | 10.1021/ac5027012 | |
dc.identifier.uri | http://hdl.handle.net/10072/172435 | |
dc.description.abstract | Quantitative and accurate detection of multiple biomarkers would allow for the rapid diagnosis and treatment of diseases induced by pathogens. Monoclonal antibodies are standard affinity reagents applied for biomarkers detection; however, their production is expensive and labor-intensive. Herein, we report on newly developed nanoyeast single-chain variable fragments (NYscFv) as an attractive alternative to monoclonal antibodies, which offers the unique advantage of a cost-effective production, stability in solution, and target-specificity. By combination of surface-enhanced Raman scattering (SERS) microspectroscopy using glass-coated, highly purified SERS nanoparticle clusters as labels, with a microfluidic device comprising multiple channels, a robust platform for the sensitive duplex detection of pathogen antigens has been developed. Highly sensitive detection for individual Entamoeba histolytica antigen EHI_115350 (limit of detection = 1 pg/mL, corresponding to 58.8 fM) and EHI_182030 (10 pg/mL, corresponding 453 fM) with high specificity has been achieved, employing the newly developed corresponding NYscFv as probe in combination with SERS microspectroscopy at a single laser excitation wavelength. Our first report on SERS-based immunoassays using the novel NYscFv affinity reagent demonstrates the flexibility of NYscFv fragments as viable alternatives to monoclonal antibodies in a range of bioassay platforms and paves the way for further applications. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | American Chemical Society | |
dc.relation.ispartofpagefrom | 9930 | |
dc.relation.ispartofpageto | 9938 | |
dc.relation.ispartofissue | 19 | |
dc.relation.ispartofjournal | Analytical Chemistry | |
dc.relation.ispartofvolume | 86 | |
dc.subject.fieldofresearch | Analytical chemistry | |
dc.subject.fieldofresearch | Analytical chemistry not elsewhere classified | |
dc.subject.fieldofresearch | Other chemical sciences | |
dc.subject.fieldofresearchcode | 3401 | |
dc.subject.fieldofresearchcode | 340199 | |
dc.subject.fieldofresearchcode | 3499 | |
dc.title | Duplex Microfluidic SERS Detection of Pathogen Antigens with Nanoyeast Single-Chain Variable Fragments | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
dcterms.license | https://pubs.acs.org/page/policy/authorchoice_termsofuse.html | |
dc.description.version | Version of Record (VoR) | |
gro.rights.copyright | © 2014 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Shiddiky, Muhammad J. | |