Show simple item record

dc.contributor.authorWang, Yuling
dc.contributor.authorRauf, Sakandar
dc.contributor.authorGrewal, Yadveer S
dc.contributor.authorSpadafora, Lauren J
dc.contributor.authorShiddiky, Muhammad JA
dc.contributor.authorCangelosi, Gerard A
dc.contributor.authorSchluecker, Sebastian
dc.contributor.authorTrau, Matt
dc.date.accessioned2018-07-27T01:30:33Z
dc.date.available2018-07-27T01:30:33Z
dc.date.issued2014
dc.identifier.issn0003-2700
dc.identifier.doi10.1021/ac5027012
dc.identifier.urihttp://hdl.handle.net/10072/172435
dc.description.abstractQuantitative and accurate detection of multiple biomarkers would allow for the rapid diagnosis and treatment of diseases induced by pathogens. Monoclonal antibodies are standard affinity reagents applied for biomarkers detection; however, their production is expensive and labor-intensive. Herein, we report on newly developed nanoyeast single-chain variable fragments (NYscFv) as an attractive alternative to monoclonal antibodies, which offers the unique advantage of a cost-effective production, stability in solution, and target-specificity. By combination of surface-enhanced Raman scattering (SERS) microspectroscopy using glass-coated, highly purified SERS nanoparticle clusters as labels, with a microfluidic device comprising multiple channels, a robust platform for the sensitive duplex detection of pathogen antigens has been developed. Highly sensitive detection for individual Entamoeba histolytica antigen EHI_115350 (limit of detection = 1 pg/mL, corresponding to 58.8 fM) and EHI_182030 (10 pg/mL, corresponding 453 fM) with high specificity has been achieved, employing the newly developed corresponding NYscFv as probe in combination with SERS microspectroscopy at a single laser excitation wavelength. Our first report on SERS-based immunoassays using the novel NYscFv affinity reagent demonstrates the flexibility of NYscFv fragments as viable alternatives to monoclonal antibodies in a range of bioassay platforms and paves the way for further applications.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.relation.ispartofpagefrom9930
dc.relation.ispartofpageto9938
dc.relation.ispartofissue19
dc.relation.ispartofjournalAnalytical Chemistry
dc.relation.ispartofvolume86
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchAnalytical chemistry not elsewhere classified
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode340199
dc.subject.fieldofresearchcode3499
dc.titleDuplex Microfluidic SERS Detection of Pathogen Antigens with Nanoyeast Single-Chain Variable Fragments
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttps://pubs.acs.org/page/policy/authorchoice_termsofuse.html
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2014 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
gro.hasfulltextFull Text
gro.griffith.authorShiddiky, Muhammad J.


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record