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  • Nano-yeast-scFv Probes on Screen-Printed Gold Electrodes for Detection of Entamoeba histolytica Antigens in a Biological Matrix

    Author(s)
    Grewal, Yadveer S
    Shiddiky, Muhammad JA
    Spadafora, Lauren J
    Cangelosi, Gerard A
    Trau, Matt
    Griffith University Author(s)
    Shiddiky, Muhammad J.
    Year published
    2014
    Metadata
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    Abstract
    The time and costs associated with monoclonal antibody production limit the potential for portable diagnostic devices to penetrate the market. Replacing the antibody with a low-cost alternate affinity reagent would reduce the costs of diagnostic development and use, and lead to new portable diagnostic devices towards many diseases. Herein, we present low-cost affinity reagents, nano-yeast–scFv, on commercially available, inexpensive, and portable screen-printed electrodes for the label-free electrochemical detection of Entamoeba histolytica cyst antigens. The biosensor was able to detect antigen at concentrations down to 10 ...
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    The time and costs associated with monoclonal antibody production limit the potential for portable diagnostic devices to penetrate the market. Replacing the antibody with a low-cost alternate affinity reagent would reduce the costs of diagnostic development and use, and lead to new portable diagnostic devices towards many diseases. Herein, we present low-cost affinity reagents, nano-yeast–scFv, on commercially available, inexpensive, and portable screen-printed electrodes for the label-free electrochemical detection of Entamoeba histolytica cyst antigens. The biosensor was able to detect antigen at concentrations down to 10 pg mL−1 in buffer with an inter-assay reproducibility of (% RSD, n=3) 4.1%. The applicability of two differently engineered nano-yeast–scFv to each specifically detect their cognant E. histolytica cyst antigens was demonstrated in a biological matrix derived from human stool. Because of the simple, inexpensive, and sensitive nature of this methodology, it may offer a low-cost alternative to immunosensors based on antibody-target recognition.
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    Journal Title
    Biosensors and Bioelectronics
    Volume
    55
    DOI
    https://doi.org/10.1016/j.bios.2013.12.043
    Subject
    Analytical chemistry
    Analytical chemistry not elsewhere classified
    Biomedical engineering
    Nanotechnology
    Publication URI
    http://hdl.handle.net/10072/172437
    Collection
    • Journal articles

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