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  • Label-Free Electrochemical Detection of an Entamoeba histolytica Antigen Using Cell-Free Yeast-scFv Probes

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    Accepted Manuscript (AM)
    Author(s)
    Grewal, Yadveer S
    Shiddiky, Muhammad JA
    Gray, Sean A
    Weigel, Kris M
    Cangelosi, Gerard A
    Trau, Matt
    Griffith University Author(s)
    Shiddiky, Muhammad J.
    Year published
    2013
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    Abstract
    Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (yeast-scFv) as novel affinity reagents.Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (yeast-scFv) as novel affinity reagents.
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    Journal Title
    Chemical Communications
    Volume
    49
    Issue
    15
    DOI
    https://doi.org/10.1039/c2cc38882k
    Copyright Statement
    © 2013 Royal Society of Chemistry. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
    Subject
    Chemical sciences
    Analytical chemistry not elsewhere classified
    Publication URI
    http://hdl.handle.net/10072/172450
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    • Journal articles

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