Label-Free Electrochemical Detection of an Entamoeba histolytica Antigen Using Cell-Free Yeast-scFv Probes

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Author(s)
Grewal, Yadveer S
Shiddiky, Muhammad JA
Gray, Sean A
Weigel, Kris M
Cangelosi, Gerard A
Trau, Matt
Griffith University Author(s)
Year published
2013
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Abstract
Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (yeast-scFv) as novel affinity reagents.
Journal Title
Chemical Communications
Volume
49
Issue
15
DOI
Copyright Statement
© 2013 Royal Society of Chemistry. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
Subject
Chemical sciences
Analytical chemistry not elsewhere classified
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