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  • An Electrochemical Immunosensor to Minimize the Nonspecific Adsorption and to Improve Sensitivity of Protein Assays in Human Serum

    Author(s)
    Shiddiky, Muhammad JA
    Kithva, Prakash H
    Kozak, Darby
    Trau, Matt
    Griffith University Author(s)
    Shiddiky, Muhammad J.
    Year published
    2012
    Metadata
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    Abstract
    An electrochemical immunoassay which minimizes nonspecific protein adsorption and improves detection sensitivity of proteomic cancer biomarker is described. Our technique comprises two novel features: (i) a high density terminally functionalized poly(N-isopropyl acrylamide) ‘brush’ layer is grown by surface initiated reversible addition fragmentation chain transfer (RAFT) polymerization method from the electrode surface in order to minimize nonspecific adsorption of serum proteins and other biomolecules, and (ii) a signal amplifying ‘bionanoconjugate’ comprised of graphene oxide nanosheets decorated with CdSe quantum dots ...
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    An electrochemical immunoassay which minimizes nonspecific protein adsorption and improves detection sensitivity of proteomic cancer biomarker is described. Our technique comprises two novel features: (i) a high density terminally functionalized poly(N-isopropyl acrylamide) ‘brush’ layer is grown by surface initiated reversible addition fragmentation chain transfer (RAFT) polymerization method from the electrode surface in order to minimize nonspecific adsorption of serum proteins and other biomolecules, and (ii) a signal amplifying ‘bionanoconjugate’ comprised of graphene oxide nanosheets decorated with CdSe quantum dots and recombinant single-chain variable fragments towards MSLN, is used to ‘physically’ amplify the anodic stripping voltammetric signal. This method enabled a detection limit of ca. 1 pg/mL MSLN (RSD=4.6%, n=4) spiked in serum samples. Because of the simple, specific and sensitive nature of this methodology, we feel that it may find potential use in serum-based protein diagnostics.
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    Journal Title
    Biosensors and Bioelectronics
    Volume
    38
    Issue
    1
    DOI
    https://doi.org/10.1016/j.bios.2012.05.014
    Subject
    Analytical chemistry
    Analytical chemistry not elsewhere classified
    Biomedical engineering
    Nanotechnology
    Publication URI
    http://hdl.handle.net/10072/172453
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    • Journal articles

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