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dc.contributor.authorShiddiky, Muhammad JA
dc.contributor.authorKithva, Prakash H
dc.contributor.authorKozak, Darby
dc.contributor.authorTrau, Matt
dc.date.accessioned2018-02-26T04:07:34Z
dc.date.available2018-02-26T04:07:34Z
dc.date.issued2012
dc.identifier.issn0956-5663
dc.identifier.doi10.1016/j.bios.2012.05.014
dc.identifier.urihttp://hdl.handle.net/10072/172453
dc.description.abstractAn electrochemical immunoassay which minimizes nonspecific protein adsorption and improves detection sensitivity of proteomic cancer biomarker is described. Our technique comprises two novel features: (i) a high density terminally functionalized poly(N-isopropyl acrylamide) ‘brush’ layer is grown by surface initiated reversible addition fragmentation chain transfer (RAFT) polymerization method from the electrode surface in order to minimize nonspecific adsorption of serum proteins and other biomolecules, and (ii) a signal amplifying ‘bionanoconjugate’ comprised of graphene oxide nanosheets decorated with CdSe quantum dots and recombinant single-chain variable fragments towards MSLN, is used to ‘physically’ amplify the anodic stripping voltammetric signal. This method enabled a detection limit of ca. 1 pg/mL MSLN (RSD=4.6%, n=4) spiked in serum samples. Because of the simple, specific and sensitive nature of this methodology, we feel that it may find potential use in serum-based protein diagnostics.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom132
dc.relation.ispartofpageto137
dc.relation.ispartofissue1
dc.relation.ispartofjournalBiosensors and Bioelectronics
dc.relation.ispartofvolume38
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchAnalytical chemistry not elsewhere classified
dc.subject.fieldofresearchBiomedical engineering
dc.subject.fieldofresearchNanotechnology
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode340199
dc.subject.fieldofresearchcode4003
dc.subject.fieldofresearchcode4018
dc.titleAn Electrochemical Immunosensor to Minimize the Nonspecific Adsorption and to Improve Sensitivity of Protein Assays in Human Serum
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorShiddiky, Muhammad J.


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