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  • Graphene/Quantum Dot Bionanoconjugates as Signal Amplifiers in Stripping Voltammetric Detection of EpCAM Biomarkers

    Author(s)
    Shiddiky, Muhammad JA
    Rauf, Sakandar
    Kithva, Prakash H
    Trau, Matt
    Griffith University Author(s)
    Shiddiky, Muhammad J.
    Year published
    2012
    Metadata
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    Abstract
    A sensitive electrochemical immunosensor for the detection of epithelial cell adhesion molecule (EpCAM) antigen, a common marker for tumors of epithelial origin, employing bionanoconjugates as signal-transduction labels has been developed. The bionanoconjugates were fabricated by carboxylation of the two-dimensional graphene oxide nanosheets (GRs) and immobilizing streptavidin and amine-functionalized CdSe quantum dots (QDs) on carboxylated GRs via carbodiimide coupling chemistry, followed by the immunoreaction with the biotinylated secondary antibodies. Since carboxylated GRs have a higher density of active sites, it allows ...
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    A sensitive electrochemical immunosensor for the detection of epithelial cell adhesion molecule (EpCAM) antigen, a common marker for tumors of epithelial origin, employing bionanoconjugates as signal-transduction labels has been developed. The bionanoconjugates were fabricated by carboxylation of the two-dimensional graphene oxide nanosheets (GRs) and immobilizing streptavidin and amine-functionalized CdSe quantum dots (QDs) on carboxylated GRs via carbodiimide coupling chemistry, followed by the immunoreaction with the biotinylated secondary antibodies. Since carboxylated GRs have a higher density of active sites, it allows a large number of CdSe QDs to be immobilized onto the surface of the bionanoconjugates, and hence, enhance the sensitivity of the immunosensor. The method enabled detection limits of 100 fg/mL and 1 pg/mL (based on the S/N = 3) in PBS buffer and serum samples, respectively, using anodic stripping voltammetric readout. The immunosensor showed a good selectivity, reproducibility, and long-storage stability, and may become a promising technique for the early detection of tumor biomarker in clinical/biological samples.
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    Journal Title
    Biosensors and Bioelectronics
    Volume
    35
    Issue
    1
    DOI
    https://doi.org/10.1016/j.bios.2012.02.057
    Subject
    Analytical chemistry
    Analytical chemistry not elsewhere classified
    Biomedical engineering
    Nanotechnology
    Publication URI
    http://hdl.handle.net/10072/172456
    Collection
    • Journal articles

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