dc.contributor.author | Shiddiky, Muhammad JA | |
dc.contributor.author | Rauf, Sakandar | |
dc.contributor.author | Kithva, Prakash H | |
dc.contributor.author | Trau, Matt | |
dc.date.accessioned | 2018-02-26T04:18:41Z | |
dc.date.available | 2018-02-26T04:18:41Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 0956-5663 | |
dc.identifier.doi | 10.1016/j.bios.2012.02.057 | |
dc.identifier.uri | http://hdl.handle.net/10072/172456 | |
dc.description.abstract | A sensitive electrochemical immunosensor for the detection of epithelial cell adhesion molecule (EpCAM) antigen, a common marker for tumors of epithelial origin, employing bionanoconjugates as signal-transduction labels has been developed. The bionanoconjugates were fabricated by carboxylation of the two-dimensional graphene oxide nanosheets (GRs) and immobilizing streptavidin and amine-functionalized CdSe quantum dots (QDs) on carboxylated GRs via carbodiimide coupling chemistry, followed by the immunoreaction with the biotinylated secondary antibodies. Since carboxylated GRs have a higher density of active sites, it allows a large number of CdSe QDs to be immobilized onto the surface of the bionanoconjugates, and hence, enhance the sensitivity of the immunosensor. The method enabled detection limits of 100 fg/mL and 1 pg/mL (based on the S/N = 3) in PBS buffer and serum samples, respectively, using anodic stripping voltammetric readout. The immunosensor showed a good selectivity, reproducibility, and long-storage stability, and may become a promising technique for the early detection of tumor biomarker in clinical/biological samples. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Elsevier | |
dc.relation.ispartofpagefrom | 251 | |
dc.relation.ispartofpageto | 257 | |
dc.relation.ispartofissue | 1 | |
dc.relation.ispartofjournal | Biosensors and Bioelectronics | |
dc.relation.ispartofvolume | 35 | |
dc.subject.fieldofresearch | Analytical chemistry | |
dc.subject.fieldofresearch | Analytical chemistry not elsewhere classified | |
dc.subject.fieldofresearch | Biomedical engineering | |
dc.subject.fieldofresearch | Nanotechnology | |
dc.subject.fieldofresearchcode | 3401 | |
dc.subject.fieldofresearchcode | 340199 | |
dc.subject.fieldofresearchcode | 4003 | |
dc.subject.fieldofresearchcode | 4018 | |
dc.title | Graphene/Quantum Dot Bionanoconjugates as Signal Amplifiers in Stripping Voltammetric Detection of EpCAM Biomarkers | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Shiddiky, Muhammad J. | |