Show simple item record

dc.contributor.authorShiddiky, Muhammad JA
dc.contributor.authorRauf, Sakandar
dc.contributor.authorKithva, Prakash H
dc.contributor.authorTrau, Matt
dc.date.accessioned2018-02-26T04:18:41Z
dc.date.available2018-02-26T04:18:41Z
dc.date.issued2012
dc.identifier.issn0956-5663
dc.identifier.doi10.1016/j.bios.2012.02.057
dc.identifier.urihttp://hdl.handle.net/10072/172456
dc.description.abstractA sensitive electrochemical immunosensor for the detection of epithelial cell adhesion molecule (EpCAM) antigen, a common marker for tumors of epithelial origin, employing bionanoconjugates as signal-transduction labels has been developed. The bionanoconjugates were fabricated by carboxylation of the two-dimensional graphene oxide nanosheets (GRs) and immobilizing streptavidin and amine-functionalized CdSe quantum dots (QDs) on carboxylated GRs via carbodiimide coupling chemistry, followed by the immunoreaction with the biotinylated secondary antibodies. Since carboxylated GRs have a higher density of active sites, it allows a large number of CdSe QDs to be immobilized onto the surface of the bionanoconjugates, and hence, enhance the sensitivity of the immunosensor. The method enabled detection limits of 100 fg/mL and 1 pg/mL (based on the S/N = 3) in PBS buffer and serum samples, respectively, using anodic stripping voltammetric readout. The immunosensor showed a good selectivity, reproducibility, and long-storage stability, and may become a promising technique for the early detection of tumor biomarker in clinical/biological samples.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom251
dc.relation.ispartofpageto257
dc.relation.ispartofissue1
dc.relation.ispartofjournalBiosensors and Bioelectronics
dc.relation.ispartofvolume35
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchAnalytical chemistry not elsewhere classified
dc.subject.fieldofresearchBiomedical engineering
dc.subject.fieldofresearchNanotechnology
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode340199
dc.subject.fieldofresearchcode4003
dc.subject.fieldofresearchcode4018
dc.titleGraphene/Quantum Dot Bionanoconjugates as Signal Amplifiers in Stripping Voltammetric Detection of EpCAM Biomarkers
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorShiddiky, Muhammad J.


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record