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  • Facile synthesis of pH-sensitive germanium nanocrystals with high quantum yield for intracellular acidic compartment imaging

    Author(s)
    Li, Feng
    Wang, Jing
    Sun, Shuqing
    Wang, Hai
    Tang, Zhiyong
    Nie, Guangjun
    Griffith University Author(s)
    Tang, Zhiyong
    Year published
    2015
    Metadata
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    Abstract
    A green-light emitting germanium nanocrystal-based biosensor to monitor lysosomal pH changes is developed. The Ge nanocrystals are synthesized in an aqueous solution with a significantly enhanced photoluminescence quantum yield of 26%. This synthesis involves a facile solution based route which avoided the use of toxic or environmentally unfriendly agents. Importantly, the photoluminescence intensity of the synthesized Ge nanocrystals is particularly sensitive to changes in pH between 5 and 6. When incubated with cultured cells, the nanocrystals are internalized and subsequently translocated via the lysosomal pathway, and ...
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    A green-light emitting germanium nanocrystal-based biosensor to monitor lysosomal pH changes is developed. The Ge nanocrystals are synthesized in an aqueous solution with a significantly enhanced photoluminescence quantum yield of 26%. This synthesis involves a facile solution based route which avoided the use of toxic or environmentally unfriendly agents. Importantly, the photoluminescence intensity of the synthesized Ge nanocrystals is particularly sensitive to changes in pH between 5 and 6. When incubated with cultured cells, the nanocrystals are internalized and subsequently translocated via the lysosomal pathway, and the Ge nanocrystals' fluorescence are greatly enhanced, even when the lysosomal pH is only slightly increased. These results reveal that the Ge nanocrystals possess high pH sensitivity compared to a commercially available dye, LysoSensor Green DND-189. The fluorescent properties of the Ge nanocrystals are demonstrated to be dependent on both the crystal form and their surface chemistry. The superior fluorescence properties and bioapplicability of the Ge nanocrystals makes them a promising intracellular bioimaging probe for monitoring various pH-sensitive processes in cells.
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    Journal Title
    Small
    Volume
    11
    Issue
    16
    DOI
    https://doi.org/10.1002/smll.201402743
    Subject
    Nanotechnology not elsewhere classified
    Publication URI
    http://hdl.handle.net/10072/172972
    Collection
    • Journal articles

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