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dc.contributor.authorLi, Feng
dc.contributor.authorWang, Jing
dc.contributor.authorSun, Shuqing
dc.contributor.authorWang, Hai
dc.contributor.authorTang, Zhiyong
dc.contributor.authorNie, Guangjun
dc.date.accessioned2017-11-03T05:01:58Z
dc.date.available2017-11-03T05:01:58Z
dc.date.issued2015
dc.identifier.issn1613-6810
dc.identifier.doi10.1002/smll.201402743
dc.identifier.urihttp://hdl.handle.net/10072/172972
dc.description.abstractA green-light emitting germanium nanocrystal-based biosensor to monitor lysosomal pH changes is developed. The Ge nanocrystals are synthesized in an aqueous solution with a significantly enhanced photoluminescence quantum yield of 26%. This synthesis involves a facile solution based route which avoided the use of toxic or environmentally unfriendly agents. Importantly, the photoluminescence intensity of the synthesized Ge nanocrystals is particularly sensitive to changes in pH between 5 and 6. When incubated with cultured cells, the nanocrystals are internalized and subsequently translocated via the lysosomal pathway, and the Ge nanocrystals' fluorescence are greatly enhanced, even when the lysosomal pH is only slightly increased. These results reveal that the Ge nanocrystals possess high pH sensitivity compared to a commercially available dye, LysoSensor Green DND-189. The fluorescent properties of the Ge nanocrystals are demonstrated to be dependent on both the crystal form and their surface chemistry. The superior fluorescence properties and bioapplicability of the Ge nanocrystals makes them a promising intracellular bioimaging probe for monitoring various pH-sensitive processes in cells.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherJohn Wiley & Sons
dc.relation.ispartofpagefrom1954
dc.relation.ispartofpageto1961
dc.relation.ispartofissue16
dc.relation.ispartofjournalSmall
dc.relation.ispartofvolume11
dc.subject.fieldofresearchNanotechnology not elsewhere classified
dc.subject.fieldofresearchcode401899
dc.titleFacile synthesis of pH-sensitive germanium nanocrystals with high quantum yield for intracellular acidic compartment imaging
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorTang, Zhiyong


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