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dc.contributor.authorTai, Anna Sze
dc.contributor.authorBell, Scott Cameron
dc.contributor.authorKidd, Timothy James
dc.contributor.authorTrembizki, Ella
dc.contributor.authorBuckley, Cameron
dc.contributor.authorRamsay, Kay Annette
dc.contributor.authorDavid, Michael
dc.contributor.authorWainwright, Claire Elizabeth
dc.contributor.authorGrimwood, Keith
dc.contributor.authorWhiley, David Mark
dc.date.accessioned2018-01-19T05:24:26Z
dc.date.available2018-01-19T05:24:26Z
dc.date.issued2015
dc.identifier.issn1932-6203
dc.identifier.doi10.1371/journal.pone.0144022
dc.identifier.urihttp://hdl.handle.net/10072/173004
dc.description.abstractIn cystic fibrosis (CF), Pseudomonas aeruginosa undergoes intra-strain genotypic and phenotypic diversification while establishing and maintaining chronic lung infections. As the clinical significance of these changes is uncertain, we investigated intra-strain diversity in commonly shared strains fromCF patients to determine if specific gene mutations were associated with increased antibiotic resistance and worse clinical outcomes. Two-hundred-and-one P. aeruginosa isolates (163 represented a dominant Australian shared strain, AUST-02) from two Queensland CF centres over two distinct time-periods (2001–2002 and 2007–2009) underwent mexZ and lasR sequencing. Broth microdilution antibiotic susceptibility testing in a subset of isolates was also performed. We identified a novel AUST-02 subtype (M3L7) in adults attending a single Queensland CF centre. This M3L7 subtype was multi-drug resistant and had significantly higher antibiotic minimum inhibitory concentrations than other AUST-02 subtypes. Prospective molecular surveillance using polymerase chain reaction assays determined the prevalence of the ‘M3L7’ subtype at this centre during 2007–2009 (170 patients) and 2011 (173 patients). Three-year clinical outcomes of patients harbouring different strains and subtypes were compared. MexZ and LasR sequences from AUST-02 isolates were more likely in 2007–2009 than 2001–2002 to exhibit mutations (mexZ: odds ratio (OR) = 3.8; 95% confidence interval (CI): 1.1–13.5 and LasR: OR = 2.5; 95%CI: 1.3–5.0). Surveillance at the adult centre in 2007–2009 identified M3L7 in 28/509 (5.5%) P. aeruginosa isolates from 13/ 170 (7.6%) patients. A repeat survey in 2011 identifiedM3L7 in 21/519 (4.0%) P. aeruginosa isolates from 11/173 (6.4%) patients. The M3L7 subtype was associated with greater intravenous antibiotic and hospitalisation requirements, and a higher 3-year risk of death/lung transplantation, than other AUST-02 subtypes (adjusted hazard ratio [HR] = 9.4; 95%CI: 2.2– 39.2) and non-AUST-02 strains (adjusted HR = 4.8; 95%CI: 1.4–16.2). This suggests ongoing microevolution of the shared CF strain, AUST-02, was associated with an emergingmultidrug resistant subtype and possibly poorer clinical outcomes.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherPublic Library of Sciences
dc.relation.ispartofpagefrome0144022-1
dc.relation.ispartofpagetoe0144022-17
dc.relation.ispartofissue12
dc.relation.ispartofjournalPLoS One
dc.relation.ispartofvolume10
dc.subject.fieldofresearchClinical Sciences not elsewhere classified
dc.subject.fieldofresearchcode110399
dc.titleGenotypic diversity within a single Pseudomonas aeruginosa strain commonly shared by Australian patients with cystic fibrosis
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2015 Tai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
gro.hasfulltextFull Text
gro.griffith.authorGrimwood, Keith
gro.griffith.authorDavid, Michael


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