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dc.contributor.authorBrittan, Jane L
dc.contributor.authorSprague, Susan V
dc.contributor.authorMacdonald, Emma L
dc.contributor.authorLove, Robert M
dc.contributor.authorJenkinson, Howard F
dc.contributor.authorWest, Nicola X
dc.date.accessioned2018-01-21T22:03:23Z
dc.date.available2018-01-21T22:03:23Z
dc.date.issued2016
dc.identifier.issn1678-7757
dc.identifier.doi10.1590/1678-775720150448
dc.identifier.urihttp://hdl.handle.net/10072/173082
dc.description.abstractObjective Bacterial penetration of dentinal tubules via exposed dentine can lead to root caries and promote infections of the pulp and root canal system. The aim of this work was to develop a new experimental model for studying bacterial invasion of dentinal tubules within the human oral cavity. Material and Methods Sections of human root dentine were mounted into lower oral appliances that were worn by four human subjects for 15 d. Roots were then fixed, sectioned, stained and examined microscopically for evidence of bacterial invasion. Levels of invasion were expressed as Tubule Invasion Factor (TIF). DNA was extracted from root samples, subjected to polymerase chain reaction amplification of 16S rRNA genes, and invading bacteria were identified by comparison of sequences with GenBank database. Results All root dentine samples with patent tubules showed evidence of bacterial cell invasion (TIF value range from 5.7 to 9.0) to depths of 200 mm or more. A spectrum of Gram-positive and Gram-negative cell morphotypes were visualized, and molecular typing identified species of Granulicatella, Streptococcus, Klebsiella, Enterobacter, Acinetobacter, and Pseudomonas as dentinal tubule residents. Conclusion A novel in vivo model is described, which provides for human root dentine to be efficiently infected by oral microorganisms. A range of bacteria were able to initially invade dentinal tubules within exposed dentine. The model will be useful for testing the effectiveness of antiseptics, irrigants, and potential tubule occluding agents in preventing bacterial invasion of dentine.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherUniversidade de Sao Paulo
dc.relation.ispartofpagefrom126
dc.relation.ispartofpageto135
dc.relation.ispartofissue2
dc.relation.ispartofjournalJournal of Applied Oral Science
dc.relation.ispartofvolume24
dc.subject.fieldofresearchDentistry not elsewhere classified
dc.subject.fieldofresearchDentistry
dc.subject.fieldofresearchcode110599
dc.subject.fieldofresearchcode1105
dc.titleIn vivo model for microbial invasion of tooth root dentinal tubules
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/deed.en
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2016 This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorLove, Robert M.


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