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dc.contributor.authorJohnson, Kenneth A
dc.contributor.authorVe, Thomas
dc.contributor.authorLarsen, Oivind
dc.contributor.authorPedersen, Rolf B
dc.contributor.authorLillehaug, Johan R
dc.contributor.authorJensen, Harald B
dc.contributor.authorHelland, Ronny
dc.contributor.authorKarlsen, Odd A
dc.date.accessioned2018-01-19T05:15:04Z
dc.date.available2018-01-19T05:15:04Z
dc.date.issued2014
dc.identifier.issn1932-6203
dc.identifier.doi10.1371/journal.pone.0087750
dc.identifier.urihttp://hdl.handle.net/10072/173105
dc.description.abstractCorA is a copper repressible protein previously identified in the methanotrophic bacterium Methylomicrobium album BG8. In this work, we demonstrate that CorA is located on the cell surface and binds one copper ion per proteinmolecule, which, based on X-ray Absorption Near Edge Structure analysis, is in the reduced state (Cu(I)). The structure of endogenously expressed CorA was solved using X-ray crystallography. The 1.6 A° three-dimensional structure confirmed the binding of copper and revealed that the copper atom was coordinated in a mononuclear binding site defined by two histidines, one water molecule, and the tryptophan metabolite, kynurenine. This arrangement of the copper-binding site is similar to that of its homologous protein MopE* from Metylococcus capsulatus Bath, confirming the importance of kynurenine for copper binding in these proteins. Our findings show that CorA has an overall fold similar to MopE, including the unique copper(I)-binding site and most of the secondary structure elements. We suggest that CorA plays a role in the M. album BG8 copper acquisition.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherPublic Library of Sciences
dc.relation.ispartofpagefrome87750-1
dc.relation.ispartofpagetoe87750-12
dc.relation.ispartofissue2
dc.relation.ispartofjournalPLoS One
dc.relation.ispartofvolume9
dc.subject.fieldofresearchBiochemistry and Cell Biology not elsewhere classified
dc.subject.fieldofresearchcode060199
dc.titleCorA Is a Copper Repressible Surface-Associated Copper(I)-Binding Protein Produced in Methylomicrobium album BG8
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2014 Johnson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
gro.hasfulltextFull Text
gro.griffith.authorVe, Thomas


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