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  • Extending the viability of acute brain slices

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    BuskilaPUB328.pdf (906.2Kb)
    Author(s)
    Buskila, Yossi
    Breen, Paul P.
    Tapson, Jonathon
    van Schaik, Andre
    Barton, M.
    Morley, John
    Griffith University Author(s)
    Barton, Matthew J.
    Year published
    2014
    Metadata
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    Abstract
    The lifespan of an acute brain slice is approximately 6–12 hours, limiting potential experimentation time. We have designed a new recovery incubation system capable of extending their lifespan to more than 36 hours. This system controls the temperature of the incubated artificial cerebral spinal fluid (aCSF) while continuously passing the fluid through a UVC filtration system and simultaneously monitoring temperature and pH. The combination of controlled temperature and UVC filtering maintains bacteria levels in the lag phase and leads to the dramatic extension of the brain slice lifespan. Brain slice viability was validated ...
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    The lifespan of an acute brain slice is approximately 6–12 hours, limiting potential experimentation time. We have designed a new recovery incubation system capable of extending their lifespan to more than 36 hours. This system controls the temperature of the incubated artificial cerebral spinal fluid (aCSF) while continuously passing the fluid through a UVC filtration system and simultaneously monitoring temperature and pH. The combination of controlled temperature and UVC filtering maintains bacteria levels in the lag phase and leads to the dramatic extension of the brain slice lifespan. Brain slice viability was validated through electrophysiological recordings as well as live/dead cell assays. This system benefits researchers by monitoring incubation conditions and standardizing this artificial environment. It further provides viable tissue for two experimental days, reducing the time spent preparing brain slices and the number of animals required for research.
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    Journal Title
    Scientific Reports
    Volume
    4
    DOI
    https://doi.org/10.1038/srep05309
    Copyright Statement
    © The Author(s). 2014. This work is licensed under a Creative Commons Attribution-NonCommercial- NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http:// creativecommons.org/licenses/by-nc-nd/4.0/
    Subject
    Central Nervous System
    Publication URI
    http://hdl.handle.net/10072/173136
    Collection
    • Journal articles

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