Extending the viability of acute brain slices

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Author(s)
Buskila, Yossi
Breen, Paul P.
Tapson, Jonathon
van Schaik, Andre
Barton, M.
Morley, John
Griffith University Author(s)
Year published
2014
Metadata
Show full item recordAbstract
The lifespan of an acute brain slice is approximately 6–12 hours, limiting potential experimentation time.
We have designed a new recovery incubation system capable of extending their lifespan to more than
36 hours. This system controls the temperature of the incubated artificial cerebral spinal fluid (aCSF) while
continuously passing the fluid through a UVC filtration system and simultaneously monitoring
temperature and pH. The combination of controlled temperature and UVC filtering maintains bacteria
levels in the lag phase and leads to the dramatic extension of the brain slice lifespan. Brain slice viability was
validated ...
View more >The lifespan of an acute brain slice is approximately 6–12 hours, limiting potential experimentation time. We have designed a new recovery incubation system capable of extending their lifespan to more than 36 hours. This system controls the temperature of the incubated artificial cerebral spinal fluid (aCSF) while continuously passing the fluid through a UVC filtration system and simultaneously monitoring temperature and pH. The combination of controlled temperature and UVC filtering maintains bacteria levels in the lag phase and leads to the dramatic extension of the brain slice lifespan. Brain slice viability was validated through electrophysiological recordings as well as live/dead cell assays. This system benefits researchers by monitoring incubation conditions and standardizing this artificial environment. It further provides viable tissue for two experimental days, reducing the time spent preparing brain slices and the number of animals required for research.
View less >
View more >The lifespan of an acute brain slice is approximately 6–12 hours, limiting potential experimentation time. We have designed a new recovery incubation system capable of extending their lifespan to more than 36 hours. This system controls the temperature of the incubated artificial cerebral spinal fluid (aCSF) while continuously passing the fluid through a UVC filtration system and simultaneously monitoring temperature and pH. The combination of controlled temperature and UVC filtering maintains bacteria levels in the lag phase and leads to the dramatic extension of the brain slice lifespan. Brain slice viability was validated through electrophysiological recordings as well as live/dead cell assays. This system benefits researchers by monitoring incubation conditions and standardizing this artificial environment. It further provides viable tissue for two experimental days, reducing the time spent preparing brain slices and the number of animals required for research.
View less >
Journal Title
Scientific Reports
Volume
4
Copyright Statement
© The Author(s). 2014. This work is licensed under a Creative Commons Attribution-NonCommercial-
NoDerivs 4.0 International License. The images or other third party material in
this article are included in the article’s Creative Commons license, unless indicated
otherwise in the credit line; if the material is not included under the Creative
Commons license, users will need to obtain permission from the license holder
in order to reproduce the material. To view a copy of this license, visit http://
creativecommons.org/licenses/by-nc-nd/4.0/
Subject
Central Nervous System