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dc.contributor.authorBuskila, Yossi
dc.contributor.authorBreen, Paul P.
dc.contributor.authorTapson, Jonathon
dc.contributor.authorvan Schaik, Andre
dc.contributor.authorBarton, M.
dc.contributor.authorMorley, John
dc.date.accessioned2018-01-18T04:19:15Z
dc.date.available2018-01-18T04:19:15Z
dc.date.issued2014
dc.identifier.issn2045-2322
dc.identifier.doi10.1038/srep05309
dc.identifier.urihttp://hdl.handle.net/10072/173136
dc.description.abstractThe lifespan of an acute brain slice is approximately 6–12 hours, limiting potential experimentation time. We have designed a new recovery incubation system capable of extending their lifespan to more than 36 hours. This system controls the temperature of the incubated artificial cerebral spinal fluid (aCSF) while continuously passing the fluid through a UVC filtration system and simultaneously monitoring temperature and pH. The combination of controlled temperature and UVC filtering maintains bacteria levels in the lag phase and leads to the dramatic extension of the brain slice lifespan. Brain slice viability was validated through electrophysiological recordings as well as live/dead cell assays. This system benefits researchers by monitoring incubation conditions and standardizing this artificial environment. It further provides viable tissue for two experimental days, reducing the time spent preparing brain slices and the number of animals required for research.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Macmillan
dc.relation.ispartofpagefrom5309-1
dc.relation.ispartofpageto5309-7
dc.relation.ispartofjournalScientific Reports
dc.relation.ispartofvolume4
dc.subject.fieldofresearchCentral Nervous System
dc.subject.fieldofresearchcode110903
dc.titleExtending the viability of acute brain slices
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s). 2014. This work is licensed under a Creative Commons Attribution-NonCommercial- NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http:// creativecommons.org/licenses/by-nc-nd/4.0/
gro.hasfulltextFull Text
gro.griffith.authorBarton, Matthew J.


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