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dc.contributor.authorHamilton, P.B.
dc.contributor.authorUren Webster, T.M.
dc.contributor.authorBasiewicz, M.
dc.contributor.authorKennedy, Emma V.
dc.contributor.authorDe-Bastos, E.S.R.
dc.contributor.authorMathews, F.
dc.date.accessioned2018-10-04T23:14:35Z
dc.date.available2018-10-04T23:14:35Z
dc.date.issued2015
dc.identifier.issn1877-7260
dc.identifier.doi10.1007/s12686-015-0470-2
dc.identifier.urihttp://hdl.handle.net/10072/173203
dc.description.abstractThe development of a rapid method for identification of the fifteen common species of British bat is described: Barbastella barbastellus, Eptesicus serotinus, Myotis bechsteinii, Myotis daubentonii, Myotis mystacinus, Myotis nattereri, Nyctalus leisleri, Nyctalus noctula, Plecotus auritus, Plecotus austriacus, Pipistrellus nathusii, Pipistrellus pipistrellus, Pipistrellus pygmaeus, Rhinolophus ferrumequinum and Rhinolophus hipposideros. A single multiplex PCR containing five fluorescently-tagged primers and fifteen species-specific primers targeting the mitochondrial control region and genes for cytb and ND1 produces clear peaks that are unique to each species when visualised on a DNA sequencer. The multiplex also includes primers to test for PCR-amplifiable bat DNA. This method will facilitate high throughput species identification of British bats, particularly in cases where phenotypic identification is difficult.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofpagefrom651
dc.relation.ispartofpageto657
dc.relation.ispartofissue3
dc.relation.ispartofjournalConservation Genetics Resources
dc.relation.ispartofvolume7
dc.subject.fieldofresearchGenetics not elsewhere classified
dc.subject.fieldofresearchEvolutionary Biology
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchFisheries Sciences
dc.subject.fieldofresearchcode060499
dc.subject.fieldofresearchcode0603
dc.subject.fieldofresearchcode0604
dc.subject.fieldofresearchcode0704
dc.titleA rapid PCR-based test for identification of fifteen British bat species
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorKennedy, Emma


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