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  • Detection of biofilm in bronchoalveolar lavage from children with non-cystic fibrosis bronchiectasis

    Author(s)
    Marsh, Robyn L
    Thornton, Ruth B
    Smith-Vaughan, Heidi C
    Richmond, Peter
    Pizzutto, Susan J
    Chang, Anne B
    Griffith University Author(s)
    Smith-Vaughan, Heidi
    Year published
    2015
    Metadata
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    Abstract
    Background: The presence of Pseudomonas aeruginosa biofilms in lower airway specimens from cystic fibrosis (CF) patients is well established. To date, biofilm has not been demonstrated in bronchoalveolar lavage (BAL) from people with non-CF bronchiectasis. The aim of this study was to determine (i) if biofilm was present in BAL from children with and without bronchiectasis, and (ii) if biofilm detection differed between sequentially collected BAL. Methods: Testing for biofilm in two sequentially collected BAL from children with and without bronchiectasis was done using BacLightTM live–dead staining and lectin staining for ...
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    Background: The presence of Pseudomonas aeruginosa biofilms in lower airway specimens from cystic fibrosis (CF) patients is well established. To date, biofilm has not been demonstrated in bronchoalveolar lavage (BAL) from people with non-CF bronchiectasis. The aim of this study was to determine (i) if biofilm was present in BAL from children with and without bronchiectasis, and (ii) if biofilm detection differed between sequentially collected BAL. Methods: Testing for biofilm in two sequentially collected BAL from children with and without bronchiectasis was done using BacLightTM live–dead staining and lectin staining for extracellular polymeric biofilm matrices. Bacterial culture and cytological measures were performed on the first and second lavages, respectively. Clinically important BAL infection was defined as >104 cfu of respiratory pathogens/ml BAL. Results: Biofilm was detected in BAL from seven of eight (87.5%) children with bronchiectasis (aged 0.8–6.9 years), but was not detected in any of three controls (aged 1.3–8.6 years). The biofilms contained both live and dead bacteria irrespective of antibiotic use prior to bronchoscopy. Biofilm was detected more frequently in the second lavage than the first. Three of the seven biofilm-positive BAL were culture-positive for respiratory pathogens at clinically important levels. Conclusions: Biofilm is present in BAL from children with non-CF bronchiectasis even when BAL-defined clinically important infection was absent. Studies to characterize lower airway biofilms and determine how biofilm contributes to bronchiectasis disease progression and treatment outcomes are necessary.
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    Journal Title
    Pediatric Pulmonology
    Volume
    50
    Issue
    3
    DOI
    https://doi.org/10.1002/ppul.23031
    Subject
    Public Health and Health Services not elsewhere classified
    Paediatrics and Reproductive Medicine
    Publication URI
    http://hdl.handle.net/10072/173551
    Collection
    • Journal articles

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