Detection of biofilm in bronchoalveolar lavage from children with non-cystic fibrosis bronchiectasis
Author(s)
Marsh, Robyn L
Thornton, Ruth B
Smith-Vaughan, Heidi C
Richmond, Peter
Pizzutto, Susan J
Chang, Anne B
Griffith University Author(s)
Year published
2015
Metadata
Show full item recordAbstract
Background: The presence of Pseudomonas aeruginosa biofilms in lower airway
specimens from cystic fibrosis (CF) patients is well established. To date, biofilm has not been
demonstrated in bronchoalveolar lavage (BAL) from people with non-CF bronchiectasis. The aim of
this study was to determine (i) if biofilm was present in BAL from children with and without
bronchiectasis, and (ii) if biofilm detection differed between sequentially collected BAL. Methods:
Testing for biofilm in two sequentially collected BAL from children with and without bronchiectasis
was done using BacLightTM live–dead staining and lectin staining for ...
View more >Background: The presence of Pseudomonas aeruginosa biofilms in lower airway specimens from cystic fibrosis (CF) patients is well established. To date, biofilm has not been demonstrated in bronchoalveolar lavage (BAL) from people with non-CF bronchiectasis. The aim of this study was to determine (i) if biofilm was present in BAL from children with and without bronchiectasis, and (ii) if biofilm detection differed between sequentially collected BAL. Methods: Testing for biofilm in two sequentially collected BAL from children with and without bronchiectasis was done using BacLightTM live–dead staining and lectin staining for extracellular polymeric biofilm matrices. Bacterial culture and cytological measures were performed on the first and second lavages, respectively. Clinically important BAL infection was defined as >104 cfu of respiratory pathogens/ml BAL. Results: Biofilm was detected in BAL from seven of eight (87.5%) children with bronchiectasis (aged 0.8–6.9 years), but was not detected in any of three controls (aged 1.3–8.6 years). The biofilms contained both live and dead bacteria irrespective of antibiotic use prior to bronchoscopy. Biofilm was detected more frequently in the second lavage than the first. Three of the seven biofilm-positive BAL were culture-positive for respiratory pathogens at clinically important levels. Conclusions: Biofilm is present in BAL from children with non-CF bronchiectasis even when BAL-defined clinically important infection was absent. Studies to characterize lower airway biofilms and determine how biofilm contributes to bronchiectasis disease progression and treatment outcomes are necessary.
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View more >Background: The presence of Pseudomonas aeruginosa biofilms in lower airway specimens from cystic fibrosis (CF) patients is well established. To date, biofilm has not been demonstrated in bronchoalveolar lavage (BAL) from people with non-CF bronchiectasis. The aim of this study was to determine (i) if biofilm was present in BAL from children with and without bronchiectasis, and (ii) if biofilm detection differed between sequentially collected BAL. Methods: Testing for biofilm in two sequentially collected BAL from children with and without bronchiectasis was done using BacLightTM live–dead staining and lectin staining for extracellular polymeric biofilm matrices. Bacterial culture and cytological measures were performed on the first and second lavages, respectively. Clinically important BAL infection was defined as >104 cfu of respiratory pathogens/ml BAL. Results: Biofilm was detected in BAL from seven of eight (87.5%) children with bronchiectasis (aged 0.8–6.9 years), but was not detected in any of three controls (aged 1.3–8.6 years). The biofilms contained both live and dead bacteria irrespective of antibiotic use prior to bronchoscopy. Biofilm was detected more frequently in the second lavage than the first. Three of the seven biofilm-positive BAL were culture-positive for respiratory pathogens at clinically important levels. Conclusions: Biofilm is present in BAL from children with non-CF bronchiectasis even when BAL-defined clinically important infection was absent. Studies to characterize lower airway biofilms and determine how biofilm contributes to bronchiectasis disease progression and treatment outcomes are necessary.
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Journal Title
Pediatric Pulmonology
Volume
50
Issue
3
Subject
Public Health and Health Services not elsewhere classified
Paediatrics and Reproductive Medicine