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  • Diagnostic assays and sampling protocols for the detection of Batrachochytrium dendrobatidis.

    Author(s)
    Hyatt, A.
    Boyle, D.
    Olsen, V.
    Boyle, D.
    Berger, L.
    Obendorf, D.
    Dalton, A.
    Kriger, Kerry
    Hero, Jean-Marc
    Hines, H.
    Phillott, R.
    Campbell, R.
    Marantelli, C.
    Gleason, F.
    Colling, A.
    Griffith University Author(s)
    Hero, Jean-Marc
    Kriger, Kerry M.
    Year published
    2007
    Metadata
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    Abstract
    Batrachochytrium dendrobatidis is a fungus belonging to the Phylum Chytridiomycota, Class Chytridiomycetes, Order Chytridiales, and is the highly infectious aetiological agent responsible for a potentially fatal disease, chytridiomycosis, which is currently decimating many of the world's amphibian populations. The fungus infects 2 amphibian orders (Anura and Caudata), 14 families and at least 200 species and is responsible for at least 1 species extinction. Whilst the origin of the agent and routes of transmission are being debated, it has been recognised that successful management of the disease will require effective ...
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    Batrachochytrium dendrobatidis is a fungus belonging to the Phylum Chytridiomycota, Class Chytridiomycetes, Order Chytridiales, and is the highly infectious aetiological agent responsible for a potentially fatal disease, chytridiomycosis, which is currently decimating many of the world's amphibian populations. The fungus infects 2 amphibian orders (Anura and Caudata), 14 families and at least 200 species and is responsible for at least 1 species extinction. Whilst the origin of the agent and routes of transmission are being debated, it has been recognised that successful management of the disease will require effective sampling regimes and detection assays. We have developed a range of unique sampling protocols together with diagnostic assays for the detection of B. dendrobatidis in both living and deceased tadpoles and adults. Here, we formally present our data and discuss them in respect to assay sensitivity, specificity, repeatability and reproducibility. We suggest that compliance with the recommended protocols will avoid the generation of spurious results, thereby providing the international scientific and regulatory community with a set of validated procedures which will assist in the successful management of chytridiomycosis in the future.
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    Journal Title
    Diseases of Aquatic Organisms
    Volume
    73
    DOI
    https://doi.org/10.3354/dao073175
    Subject
    Biological Sciences
    Agricultural and Veterinary Sciences
    Publication URI
    http://hdl.handle.net/10072/17408
    Collection
    • Journal articles

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