Bacterial communities of soils under different forest ecosystems as revealed by molecular approaches based on 16S rDNA clone library

Abstract

A 168 rDNA clone libI'aIy was established following soil community DNA extraction, polymerase chain reaction (peR) and cloning. A total of 324 clones, including 27 from each sample collected from the natural forest (YNF), the first rotation (YIR) and the second rotation (Y2R) of hoop pine plantations (each treatments with fOill replicates) at Vanaman, in subtropical Australia, were randomly selected and sequenced to represent the bacterial composition and diversity of the clone library Phylogenetic analyses indicated that Vanaman soil had a putative bacterial cOlTIITIllility composition of Unclassified bacteria (344%), Proteobacteria (220%), Chlamydiae/ Venucomicrobia (157%), Fibrobacteres/Acidobacteria (10 2%), Chloroflexi (6.9%), Genunatimonadetes (5 6%), and Actinobacteria (5.2%). There was a significant difference among different treatments in the taxonomic group distribution Ihe soil bacterial diversity tended to decrease from the natural forest to the first and then to the second rotation ofhoop pine plantations.