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dc.contributor.convenorCanadian Physiotherapy Associationen_AU
dc.contributor.authorLaakso, Liisaen_US
dc.contributor.authorMcDonnell, Annen_US
dc.contributor.authorPowell, Katieen_US
dc.date.accessioned2017-05-03T12:57:48Z
dc.date.available2017-05-03T12:57:48Z
dc.date.issued2007en_US
dc.date.modified2008-05-01T02:51:03Z
dc.identifier.urihttp://hdl.handle.net/10072/18034
dc.description.abstractPURPOSE: This research examined the response of two breast cancer cell lines (MCF-7 and MB-435) in vitro to a range of laser doses at two commonly used wavelengths (780nm and 830nm). RELEVANCE: LLLT is being used increasingly for the management of post-mastectomy lymphoedema without undisputed evidence of its effectiveness in this disorder. In particular, information regarding dose-response relationships and safety (in malignant conditions) remains elusive. This research set out to clarify dose/response relationships in cell culture as a starting point towards making clinical recommendations for use. PARTICIPANTS: In vitro cell cultures. METHODS: Well-differentiated human breast adenocarcinoma (MCF-7) and less differentiated human breast ductal carcinoma (MB-435) cell lines were grown and passaged using standard aseptic tissue culture protocols. 96-well tissue culture plates were seeded with 1 x 104 cells per well in fresh growth medium, and allowed to grow for 48-72hrs before exposure to laser. A single dose of laser was performed at energy densities of 0.5, 1, 2.5, 10 and 12J/cm2 for 780nm laser, and 0.5, 1, 2.5, 10 and 15J/cm2 for 830nm laser. Cell proliferation was assessed 24hrs after laser treatment by calorimetric spectrophotometry. ANALYSIS: Percentage growth differences between treated and control means, and Student t-tests with 95% confidence limit were calculated. Alpha level was set at 0.05 for all tests. RESULTS: Laser irradiation at 780nm using doses from 0.5 to 5J/cm2 produced no significant difference in proliferation of MCF-7 (adenocarcinoma) cells. A positive trend in MCF-7 cell proliferation was evident at a dose of 10J/cm2 (13.37% mean percentage increase) becoming significantly increased at 12J/cm2 (p?<?0.05). In the MB-435 (ductal carcinoma) cells, laser irradiation at 780nm (doses from 0.5 to 5J/cm2) also resulted in no significant difference, but at 10J/cm2 there was a significant increase in cell proliferation compared to negative controls (p?<?0.05). At 830nm, laser irradiation using doses from 0.5 to 2.5J/cm2 did not affect MCF-7 cell proliferation, however, at higher doses of 10 and 12J/cm2, significant increases (p?<?0.05) in growth were noted. There were no effects observed in MB-435 cells after laser irradiation at 830nm for doses from 0.5 to 10J/cm2, but at 15J/cm2 there was a statistically significant increase (p?<?0.05) in cell proliferation. CONCLUSIONS: The findings suggest that 780nm and 830nm laser at doses up to 5J/cm2 do not stimulate cell proliferation in the two breast cancer cell lines studied, however higher doses do appear to stimulate cell proliferation irrespective of wavelength, and in both human breast carcinoma cell lines. We plan to investigate further the in vitro effects of other typical laser wavelengths used in lymphoedema treatment prior to extending the research to in vivo and clinical models. IMPLICATIONS: The results of this research imply that low doses of laser of less than 5J/cm2 at the wavelengths stipulated are potentially safe for clinical use in the presence of the two types of human breast cancers studied. Whilst findings from in vitro research cannot be considered transferable to clinical application, the findings provide some guidance on targeting potential foci for future work using in vivo models of treatment and in clinical applications. KEYWORDS: laser therapy, cancer. FUNDING ACKNOWLEDGEMENTS: Griffith University. CONTACT: L.Laakso@griffith.edu.auen_US
dc.description.publicationstatusYesen_AU
dc.format.extent20075 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherNo data provideden_US
dc.publisher.urihttp://www.wcpt.org/index.phpen_AU
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofconferencenameThe World Physical Therapy Congressen_US
dc.relation.ispartofconferencetitleWorld Physical Therapy 2007en_US
dc.relation.ispartofdatefrom2007-06-03en_US
dc.relation.ispartofdateto2007-06-06en_US
dc.relation.ispartoflocationVancouver, Canadaen_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchcode270199en_US
dc.titleAbstract 969: The effects of low level laser therapy (LLLT) on human breast cancer cell lines in vitro.en_US
dc.typeConference outputen_US
dc.type.descriptionE3 - Conference Publications (Extract Paper)en_US
dc.type.codeE - Conference Publicationsen_US
gro.facultyGriffith Health, School of Allied Health Sciencesen_US
gro.rights.copyrightCopyright remains with the authors 2007. The attached file is reproduced here with permission of the copyright owner[s] for your personal use only. No further distribution permitted. For information about this conference please refer to the publisher's website or contact the author[s].en_AU
gro.date.issued2007
gro.hasfulltextFull Text


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