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dc.contributor.authorAgranovski, Igoren_US
dc.contributor.authorKhromykh, Alexander A.en_US
dc.contributor.authorMokhonov, Vladen_US
dc.contributor.authorMokhonova, Ekaterinaen_US
dc.contributor.authorPyankov, Olegen_US
dc.contributor.authorPyankova, Olgaen_US
dc.contributor.authorS. Safatov, Alexanderen_US
dc.date.accessioned2017-04-04T17:08:51Z
dc.date.available2017-04-04T17:08:51Z
dc.date.issued2007en_US
dc.date.modified2009-10-09T06:12:30Z
dc.identifier.issn14622912en_US
dc.identifier.doi10.1111/j.1462-2920.2006.01226.xen_AU
dc.identifier.urihttp://hdl.handle.net/10072/18511
dc.description.abstractWe have recently developed a new personal sampler and demonstrated its feasibility for detection of viable airborne microorganisms including bacteria, fungi and viruses. To accelerate the time-consuming analytical procedure involving 2-5 days of biological testing, we employed a real-time PCR protocol in conjunction with the personal sampler for collection of airborne viruses. The advantage of this approach is that if the presence of a particular pathogen in the air is detected by the PCR, the remaining collecting liquid can be further analysed by more time-consuming biological methods to estimate the number of airborne infectious/live microorganisms. As sampling of bioaerosols in natural environments is likely to be associated with substantial contamination by a range of microorganisms commonly existing in an ambient air, an investigation of the specificity of detection by targeted PCR analysis is required. Here we present the results of the study on the detection of Influenza virus in the ambient air contaminated with high concentrations of bacteria and fungi using real-time PCR protocol. The combined sampling PCR detection method was found to be fully feasible for the rapid (~2.5 h) and highly specific (no cross-reactivity) identification of the labile airborne virus in the air containing elevated concentrations of other microorganisms.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherBlackwell Publishingen_US
dc.publisher.placeOxforden_US
dc.publisher.urihttp://www.wiley.com/bw/journal.asp?ref=1462-2912en_AU
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom992en_US
dc.relation.ispartofpageto1000en_US
dc.relation.ispartofissue4en_AU
dc.relation.ispartofjournalEnvironmental Microbiologyen_US
dc.relation.ispartofvolume9en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchcode270304en_US
dc.titleUsing a Bioaerosol Personal Sampler in combination with Real-time PCR Analysis for Rapid Detection of Airborne Virusesen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2007
gro.hasfulltextNo Full Text


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