dc.contributor.author | Prochazka, Lubomir | |
dc.contributor.author | Turanek, Jaroslav | |
dc.contributor.author | Tesarik, Radek | |
dc.contributor.author | Knotigova, Pavlina | |
dc.contributor.author | Polaskova, Pavlina | |
dc.contributor.author | Andrysik, Zdenek | |
dc.contributor.author | Kozubik, Alois | |
dc.contributor.author | Zak, Frantisek | |
dc.contributor.author | Sova, Petr | |
dc.contributor.author | Neuzil, Jiri | |
dc.contributor.author | Machala, Miroslav | |
dc.date.accessioned | 2017-05-03T12:46:25Z | |
dc.date.available | 2017-05-03T12:46:25Z | |
dc.date.issued | 2007 | |
dc.date.modified | 2009-09-09T07:17:42Z | |
dc.identifier.issn | 0003-9861 | |
dc.identifier.doi | 10.1016/j.abb.2007.03.021 | |
dc.identifier.uri | http://hdl.handle.net/10072/18792 | |
dc.description.abstract | A new hydrophobic platinum(IV) complex, LA-12, a very efficient anticancer drug lacking cross-resistance with cisplatin (CDDP), is now being tested in clinical trials. Here we investigated the apoptogenic activity of LA-12 and its effect on gap-junctional intercellular communication (GJIC) in the rat liver epithelial cell line WB-F344. LA-12 induced apoptosis much more efficiently than did CDDP due to a combination of rapid penetration into the cell and attack on DNA, leading to fast activation of p53 and caspase-3. Exposure of WB-F344 cells to LA-12 led to rapid induction of the time- and dose-dependent decrease in GJIC. On the molecular level, loss of GJIC induced by LA-12 was mediated by activation of extracellular signal-regulated kinase (ERK)-1 and ERK-2, as demonstrated by the use of inhibitors of ERK activation. Inhibition of GJIC was linked to rapid hyperphosphorylation of connexin-43 and disappearance of connexon clusters from membranes, which was not observed in the case of CDDP. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Elsevier | |
dc.publisher.place | The Netherlands | |
dc.publisher.uri | http://www.elsevier.com/wps/find/journaldescription.cws_home/622787/description#description | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofpagefrom | 54 | |
dc.relation.ispartofpageto | 61 | |
dc.relation.ispartofissue | 1 | |
dc.relation.ispartofjournal | Archives of Biochemistry and Biophysics | |
dc.relation.ispartofvolume | 462 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Biochemistry and cell biology | |
dc.subject.fieldofresearchcode | 3101 | |
dc.title | Apoptosis and inhibition of gap-junctional intercellular communication induced by LA-12, a novel hydrophobic platinum(IV) complex | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.rights.copyright | © 2007 Elsevier. Please refer to the journal's website for access to the definitive, published version. | |
gro.date.issued | 2007 | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Neuzil, Jiri | |