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dc.contributor.authorY. Hung, Daniel
dc.contributor.authorA. Siebert, Gerhard
dc.contributor.authorChang, Ping
dc.contributor.authorAnissimov, Yuri G.
dc.contributor.authorS. Roberts, Michael
dc.description.abstractThe aim of this study was to define the determinants of the linear hepatic disposition kinetics of propranolol optical isomers using a perfused rat liver. Monensin was used to abolish the lysosomal proton gradient to allow an estimation of propranolol ion trapping by hepatic acidic vesicles. In vitro studies were used for independent estimates of microsomal binding and intrinsic clearance. Hepatic extraction and mean transit time were determined from outflow-concentration profiles using a nonparametric method. Kinetic parameters were derived from a physiologically based pharmacokinetic model. Modeling showed an approximate 34-fold decrease in ion trapping following monensin treatment. The observed model-derived ion trapping was similar to estimated theoretical values. No differences in ion-trapping values was found between R(+)- and S(-)-propranolol. Hepatic propranolol extraction was sensitive to changes in liver perfusate flow, permeability-surface area product, and intrinsic clearance. Ion trapping, microsomal and nonspecific binding, and distribution of unbound propranolol accounted for 47.4, 47.1, and 5.5% of the sequestration of propranolol in the liver, respectively. It is concluded that the physiologically more active S(-)-propranolol differs from the R(+)-isomer in higher permeability-surface area product, intrinsic clearance, and intracellular binding site values.
dc.publisherThe American Society for Pharmacology and Experimental Therapeutics
dc.relation.ispartofjournalJournal of Pharmacology and Experimental Therapeutics
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences
dc.titleDisposition kinetics of propranolol isomers in the perfused rat liver
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorAnissimov, Yuri G.

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