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  • Genetic and molecular analysis of GogB, a phage-encoded type III-secreted substrate in Salmonella enterica serovar typhimurium with autonomous expression from its associated phage

    Author(s)
    K. Coombes, Brian
    E. Wickham, Mark
    F. Brown, Nat
    Lemire, Sebastien
    Bossi, Lionello
    W. L. Hsiao, William
    S. L. Brinkman, Fiona
    Brett Finlay, B.
    Griffith University Author(s)
    Brown, Nathaniel F.
    Year published
    2005
    Metadata
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    Abstract
    Salmonella enterica serovar Typhimurium is lysogenized by several temperate bacteriophages that encode lysogenic conversion genes, which can act as virulence factors during infection and contribute to the genetic diversity and pathogenic potential of the lysogen. We have investigated the temperate bacteriophage called Gifsy-1 in S. enterica serovar Typhimurium and show here that the product of the gogB gene encoded within this phage shares similarity with proteins from other Gram-negative pathogens. The amino-terminal portion of GogB shares similarity with leucine-rich repeat-containing virulence-associated proteins from ...
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    Salmonella enterica serovar Typhimurium is lysogenized by several temperate bacteriophages that encode lysogenic conversion genes, which can act as virulence factors during infection and contribute to the genetic diversity and pathogenic potential of the lysogen. We have investigated the temperate bacteriophage called Gifsy-1 in S. enterica serovar Typhimurium and show here that the product of the gogB gene encoded within this phage shares similarity with proteins from other Gram-negative pathogens. The amino-terminal portion of GogB shares similarity with leucine-rich repeat-containing virulence-associated proteins from other Gram-negative pathogens, whereas the carboxyl-terminal portion of GogB shares similarity with uncharacterized proteins in other pathogens. We show that GogB is secreted by both type III secretion systems encoded in Salmonella Pathogenicity Island-1 (SPI-1) and SPI-2 but translocation into host cells is a SPI-2-mediated process. Once translocated, GogB localizes to the cytoplasm of infected host cells. The genetic regulation of gogB in Salmonella is influenced by the transcriptional activator, SsrB, under SPI-2-inducing conditions, but the modular nature of the gogB gene allows for autonomous expression and type III secretion following horizontal gene transfer into a heterologous pathogen.
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    Journal Title
    Journal of molecular biology.
    Volume
    348
    Issue
    4
    Publisher URI
    http://www.elsevier.com/wps/find/journaldescription.cws_home/622890/description#description
    DOI
    https://doi.org/10.1016/j.jmb.2005.03.024
    Subject
    Medicinal and Biomolecular Chemistry
    Biochemistry and Cell Biology
    Microbiology
    Publication URI
    http://hdl.handle.net/10072/20464
    Collection
    • Journal articles

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