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dc.contributor.authorK. Coombes, Brian
dc.contributor.authorE. Wickham, Mark
dc.contributor.authorF. Brown, Nat
dc.contributor.authorLemire, Sebastien
dc.contributor.authorBossi, Lionello
dc.contributor.authorW. L. Hsiao, William
dc.contributor.authorS. L. Brinkman, Fiona
dc.contributor.authorBrett Finlay, B.
dc.date.accessioned2017-05-03T12:25:16Z
dc.date.available2017-05-03T12:25:16Z
dc.date.issued2005
dc.date.modified2008-11-19T02:55:17Z
dc.identifier.issn00222836
dc.identifier.doi10.1016/j.jmb.2005.03.024
dc.identifier.urihttp://hdl.handle.net/10072/20464
dc.description.abstractSalmonella enterica serovar Typhimurium is lysogenized by several temperate bacteriophages that encode lysogenic conversion genes, which can act as virulence factors during infection and contribute to the genetic diversity and pathogenic potential of the lysogen. We have investigated the temperate bacteriophage called Gifsy-1 in S. enterica serovar Typhimurium and show here that the product of the gogB gene encoded within this phage shares similarity with proteins from other Gram-negative pathogens. The amino-terminal portion of GogB shares similarity with leucine-rich repeat-containing virulence-associated proteins from other Gram-negative pathogens, whereas the carboxyl-terminal portion of GogB shares similarity with uncharacterized proteins in other pathogens. We show that GogB is secreted by both type III secretion systems encoded in Salmonella Pathogenicity Island-1 (SPI-1) and SPI-2 but translocation into host cells is a SPI-2-mediated process. Once translocated, GogB localizes to the cytoplasm of infected host cells. The genetic regulation of gogB in Salmonella is influenced by the transcriptional activator, SsrB, under SPI-2-inducing conditions, but the modular nature of the gogB gene allows for autonomous expression and type III secretion following horizontal gene transfer into a heterologous pathogen.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAcademic Press
dc.publisher.placeUnited Kingdom
dc.publisher.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/622890/description#description
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom817
dc.relation.ispartofpageto830
dc.relation.ispartofissue4
dc.relation.ispartofjournalJournal of molecular biology.
dc.relation.ispartofvolume348
dc.rights.retentionN
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchcode0304
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode0605
dc.titleGenetic and molecular analysis of GogB, a phage-encoded type III-secreted substrate in Salmonella enterica serovar typhimurium with autonomous expression from its associated phage
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2005
gro.hasfulltextNo Full Text
gro.griffith.authorBrown, Nathaniel F.


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