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  • Characterizing Embryonic Gene Expression Patterns in the Mouse Using Nonredundant Sequence-Based Selection

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    Author(s)
    Sousa-Nunes, Rita
    Rana, Amer Ahmed
    Kettleborough, Ross
    M. Brickman, Joshua
    Clements, Melanie
    Forrest, Alistair
    Grimmond, Sean
    Avner, Philip
    Smith, James
    L .Dunwoodie, Sally
    S. P. Beddington, Rosa
    Griffith University Author(s)
    Forrest, Alistair RR.
    Year published
    2003
    Metadata
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    Abstract
    This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. ...
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    This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.
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    Journal Title
    Genome Research
    Volume
    13
    Issue
    12
    DOI
    https://doi.org/10.1101/gr.1362303
    Copyright Statement
    © 2003 Cold Spring Harbor Laboratory Press. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
    Subject
    Biological Sciences
    Medical and Health Sciences
    Publication URI
    http://hdl.handle.net/10072/20586
    Collection
    • Journal articles

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