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dc.contributor.authorJ Ingram, Wendy
dc.contributor.authorWicking, Carol
dc.contributor.authorGrimmond, Sean
dc.contributor.authorForrest, Alistair
dc.contributor.authorWainwright, Brandon J.
dc.date.accessioned2017-05-03T15:01:20Z
dc.date.available2017-05-03T15:01:20Z
dc.date.issued2002
dc.date.modified2009-08-27T06:52:06Z
dc.identifier.issn09509232
dc.identifier.doi10.1038/sj.onc.1205975
dc.identifier.urihttp://hdl.handle.net/10072/20589
dc.description.abstractSonic Hedgehog is a secreted morphogen involved in patterning a wide range of structures in the developing embryo. Disruption of the Hedgehog signalling cascade leads to a number of developmental disorders and plays a key role in the formation of a range of human cancers. The identification of genes regulated by Hedgehog is crucial to understanding how disruption of this pathway leads to neoplastic transformation. We have used a Sonic Hedgehog (Shh) responsive mouse cell line, C3H/10T1/2, to provide a model system for hedgehog target gene discovery. Following activation of cell cultures with Shh, RNA was used to interrogate microarrays to investigate downstream transcriptional consequences of hedgehog stimulation. As a result 11 target genes have been identified, seven of which are induced (Thrombomodulin, GILZ, BF-2, Nr4a1, IGF2, PMP22, LASP1) and four of which are repressed (SFRP-1, SFRP-2, Mip1-, Amh) by Shh. These targets have a diverse range of putative functions and include transcriptional regulators and molecules known to be involved in regulating cell growth or apoptosis. The corroboration of genes previously implicated in hedgehog signalling, along with the finding of novel targets, demonstrates both the validity and power of the C3H/10T1/2 system for Shh target gene discovery.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Publishing Group
dc.publisher.placeUnited Kingdom
dc.publisher.urihttp://www.nature.com/onc/index.html
dc.relation.ispartofpagefrom8196
dc.relation.ispartofpageto8205
dc.relation.ispartofissue53
dc.relation.ispartofjournalOncogene
dc.relation.ispartofvolume21
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchOncology and Carcinogenesis
dc.subject.fieldofresearchcode1103
dc.subject.fieldofresearchcode1112
dc.titleNovel genes regulated by Sonic Hedgehog in pluripotent mesenchymal cells
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2002 Nature Publishing Group. Please refer to the journal's website for access to the definitive, published version.
gro.date.issued2002
gro.hasfulltextNo Full Text
gro.griffith.authorForrest, Alistair RR.


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