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  • Tissue-specific gene expression in soybean (Glycine max) detected by cDNA microarray analysis

    Author(s)
    L. Maquire, Tina
    Grimmond, Sean
    Forrest, Alistair
    Iturbe-Ormaetxe, Inaki
    Meksem, Khalid
    Gresshoff, Peter
    Griffith University Author(s)
    Forrest, Alistair RR.
    Year published
    2002
    Metadata
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    Abstract
    We have constructed cDNA microarrays for soybean (Glycine maxL. Merrill), containing approximately 4,100 Unigene ESTs derived from axenic roots, to evaluate their application and utility for functional genomics of organ differentiation in legumes. We assessed microarray technology by conducting studies to evaluate the accuracy of microarray data and have found them to be both reliable and reproducible in repeat hybridisations. Several ESTs showed high levels (50 fold) of differential expression in either root or shoot tissue of soybean. A small number of physiologically interesting, and differentially expressed sequences ...
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    We have constructed cDNA microarrays for soybean (Glycine maxL. Merrill), containing approximately 4,100 Unigene ESTs derived from axenic roots, to evaluate their application and utility for functional genomics of organ differentiation in legumes. We assessed microarray technology by conducting studies to evaluate the accuracy of microarray data and have found them to be both reliable and reproducible in repeat hybridisations. Several ESTs showed high levels (50 fold) of differential expression in either root or shoot tissue of soybean. A small number of physiologically interesting, and differentially expressed sequences found by microarray analysis were verified by both quantitative real-time RT-PCR and Northern blot analysis. There was a linear correlation (r2 = 0.99, over 5 orders of magnitude) between microarray and quantitative real-time RT-PCR data. Microarray analysis of soybean has enormous potential not only for the discovery of new genes involved in tissue differentiation and function, but also to study the expression of previously characterised genes, gene networks and gene interactions in wild-type, mutant or transgenic plants.
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    Journal Title
    Journal of Plant Physiology
    Volume
    159
    Issue
    12
    Publisher URI
    http://www.sciencedirect.com/science/journal/01761617
    DOI
    https://doi.org/10.1078/0176-1617-00858
    Subject
    Plant Biology
    Publication URI
    http://hdl.handle.net/10072/20590
    Collection
    • Journal articles

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