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dc.contributor.authorLim, HK
dc.contributor.authorPankhurst, NW
dc.contributor.authorFitzgibbon, QP
dc.date.accessioned2017-05-03T15:20:50Z
dc.date.available2017-05-03T15:20:50Z
dc.date.issued2004
dc.identifier.issn0044-8486
dc.identifier.doi10.1016/j.aquaculture.2004.06.017
dc.identifier.urihttp://hdl.handle.net/10072/20695
dc.description.abstractSpermiated male greenback flounder (Rhombosolea tapirina) were implanted with gonadotropin releasing hormone analog (GnRHa) pellets at different dosages to examine their effects on milt characteristics and plasma levels of testosterone (T), 11-ketotestosterone (11KT) and 17,20߭dihydroxy-4-pregnen-3-one (17,20ߐ). Milt and blood samples were collected for up to 28 or 42 days post-implantation (p.i.) in two separate experiments using fish that were slightly or moderately spermiated, respectively. In both experiments, fish treated with GnRHa pellets showed a consistent and significant increase in milt volume relative to controls, and the increase of milt volume was more rapid than the increase in numbers of spermatozoa. Spermatocrit (Sct) was significantly lower in GnRHa-treated fish than in controls. Plasma levels of T and 11KT were elevated at 7 and 14 days p.i. in slightly spermiated fish treated with GnRHa and elevated plasma T and 11KT levels were accompanied by increased milt volume early in the spermiation period. In contrast, there was no significant difference in plasma T levels between GnRHa-treated and control fish in fish that were moderately spermiated at the time of implant. Treatment with GnRHa tended to result in lower plasma levels of 11KT and higher levels of 17,20ߐ relative to controls, and there was a positive relationship between the elevation of plasma 17,20ߐ and the increase in milt volume in response to implantation of GnRHa pellet. Slow release GnRHa administration had no effect on sperm activity or pH and osmolality of seminal plasma in either experiment.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoen_US
dc.publisherElsevier
dc.publisher.placeSan Diego
dc.relation.ispartofpagefrom505
dc.relation.ispartofpageto516
dc.relation.ispartofissue1-4
dc.relation.ispartofjournalAquaculture
dc.relation.ispartofvolume240
dc.subject.fieldofresearchFisheries Sciences
dc.subject.fieldofresearchZoology
dc.subject.fieldofresearchcode0704
dc.subject.fieldofresearchcode0608
dc.titleEffects of slow release gonadotropin releasing hormone analog on milt characteristics and plasma levels of gonadal steroids in greenback flounder, Rhombosolea tapirina.
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2015-02-12T04:24:18Z
gro.hasfulltextNo Full Text
gro.griffith.authorPankhurst, Ned W.


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