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dc.contributor.authorLim, Han Kyuen_US
dc.contributor.authorPankhurst, Ned Williamen_US
dc.contributor.authorFitzgibbon, Quinn Patricken_US
dc.date.accessioned2017-05-03T15:20:50Z
dc.date.available2017-05-03T15:20:50Z
dc.date.issued2004en_US
dc.identifier.issn00448486en_US
dc.identifier.doi10.1016/j.aquaculture.2004.06.017en_US
dc.identifier.urihttp://hdl.handle.net/10072/20695
dc.description.abstractSpermiated male greenback flounder (Rhombosolea tapirina) were implanted with gonadotropin releasing hormone analog (GnRHa) pellets at different dosages to examine their effects on milt characteristics and plasma levels of testosterone (T), 11-ketotestosterone (11KT) and 17,20߭dihydroxy-4-pregnen-3-one (17,20ߐ). Milt and blood samples were collected for up to 28 or 42 days post-implantation (p.i.) in two separate experiments using fish that were slightly or moderately spermiated, respectively. In both experiments, fish treated with GnRHa pellets showed a consistent and significant increase in milt volume relative to controls, and the increase of milt volume was more rapid than the increase in numbers of spermatozoa. Spermatocrit (Sct) was significantly lower in GnRHa-treated fish than in controls. Plasma levels of T and 11KT were elevated at 7 and 14 days p.i. in slightly spermiated fish treated with GnRHa and elevated plasma T and 11KT levels were accompanied by increased milt volume early in the spermiation period. In contrast, there was no significant difference in plasma T levels between GnRHa-treated and control fish in fish that were moderately spermiated at the time of implant. Treatment with GnRHa tended to result in lower plasma levels of 11KT and higher levels of 17,20ߐ relative to controls, and there was a positive relationship between the elevation of plasma 17,20ߐ and the increase in milt volume in response to implantation of GnRHa pellet. Slow release GnRHa administration had no effect on sperm activity or pH and osmolality of seminal plasma in either experiment.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.languageEnglishen_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.publisher.placeSan Diegoen_US
dc.relation.ispartofpagefrom505en_US
dc.relation.ispartofpageto516en_US
dc.relation.ispartofissue1-4en_US
dc.relation.ispartofjournalAquacultureen_US
dc.relation.ispartofvolume240en_US
dc.subject.fieldofresearchcode270604en_US
dc.titleEffects of slow release gonadotropin releasing hormone analog on milt characteristics and plasma levels of gonadal steroids in greenback flounder, Rhombosolea tapirina.en_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2015-02-12T04:24:18Z
gro.hasfulltextNo Full Text


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