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dc.contributor.authorSun, B
dc.contributor.authorPankhurst, NW
dc.date.accessioned2017-05-03T15:20:52Z
dc.date.available2017-05-03T15:20:52Z
dc.date.issued2006
dc.date.modified2008-12-02T04:35:24Z
dc.identifier.issn1095-6433
dc.identifier.doi10.1016/j.cbpa.2006.02.007
dc.identifier.urihttp://hdl.handle.net/10072/20701
dc.description.abstractOvarian follicles from vitellogenic greenback flounder (Rhombosolea tapirina) were incubated in L15 medium alone, or containing human chorionic gonadotropin (hCG), dibutyryl cyclic AMP (dbcAMP) or the steroid precursors testosterone (T), 17-hydroxyprogesterone (17P) and androstenedione (A) in the presence of vitellogenin (Vtg) at 0.1-5.0 mg mL- 1. Medium concentrations of 17߭estradiol (E2) and T were measured by radioimmunoassay. HCG generally stimulated follicular E2 but not T production, whereas 17P, A and T stimulated production of E2, T, and E2 respectively. Treatment of follicles with dbcAMP inhibited follicular E2 production, but increased follicular T production at high doses. The effect of low concentrations of Vtg on follicular steroid production was variable; however, higher doses of Vtg significantly suppressed basal, hCG-, dbcAMP- and steroid precursor-stimulated follicular E2 and T production. The results of this study show that high concentrations of Vtg may suppress follicular steroid production by interfering in the steroidogenic pathway. This suggests that Vtg may regulate its own production by limiting the ovarian production of E2.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeSan Diego
dc.publisher.urihttp://www.elsevier.com/locate/cbpa
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom78
dc.relation.ispartofpageto85
dc.relation.ispartofissue1
dc.relation.ispartofjournalComparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
dc.relation.ispartofvolume144
dc.rights.retentionY
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchZoology
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3109
dc.titleIn vitro effect of vitellogenin on steroid production by ovarian follicles of greenback flounder Rhombosolea tapirina.
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2006
gro.hasfulltextNo Full Text
gro.griffith.authorPankhurst, Ned W.


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